EIF4G1 Protein (AA 1-1600) (Strep Tag)

Catalog No. ABIN3135726

Product Details

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1600

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This EIF4G1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MNKAPQPTGP PPARSPGLPQ PAFPPGQTAP VVFSTPQATQ MNTPSQPRQG GFRSLQHFYP SRAQPPSSAA SRVQSAAPAR PGPAPHVYPA GSQVMMIPSQ ISYSASQGAY YIPGQGRSTY VVPTQQYPVQ PGAPGFYPGA SPTEFGTYAG AYYPAQGVQQ FPASVAPAPV LMNQPPQIAP KRERKTIRIR DPNQGGKDIT EEIMSGARTA STPTPPQTGG SLEPQPNGES PQVAVIIRPD DRSQGAAIGG RPGLPGPEHS PGTESQPSSP SPTPSPPPIL EPGSESNLGV LSIPGDTMTT GMIPMSVEES TPISCETGEP YCLSPEPTLA EPILEVEVTL SKPIPESEFS SSPLQVSTAL VPHKVETHEP NGVIPSEDLE PEVESSTEPA PPPLSPCASE SLVPIAPTAQ PEELLNGAPS PPAVDLSPVS EPEEQAKKVS SAALASILSP APPVAPSDTS PAQEEEMEED DDDEEGGEAE SEKGGEDVPL DSTPVPAQLS QNLEVAAATQ VAVSVPKRRR KIKELNKKEA VGDLLDAFKE VDPAVPEVEN QPPTGSNPSP ESEGSMVPTQ PEETEETWDS KEDKIHNAEN IQPGEQKYEY KSDQWKPLNL EEKKRYDREF LLGFQFIFAS MQKPEGLPHI TDVVLDKANK TPLRQLDPSR LPGINCGPDF TPSFANLGRP ALSNRGPPRG GPGGELPRGP AGLGPRRSQQ GPRKETRKII SSVIMTEDIK LNKAEKAWKP SSKRTAADKD RGEEDADGSK TQDLFRRVRS ILNKLTPQMF QQLMKQVTQL AIDTEERLKG VIDLIFEKAI SEPNFSVAYA NMCRCLMALK VPTTEKPTVT VNFRKLLLNR CQKEFEKDKD DDEVFEKKQK EMDEAATAEE RGRLKEELEE ARDIARRRSL GNIKFIGELF KLKMLTEAIM HDCVVKLLKN HDEESLECLC RLLTTIGKDL DFAKAKPRMD QYFNQMEKII KEKKTSSRIR FMLQDVLDLR QSNWVPRRGD QGPKTIDQIH KEAEMEEHRE HIKVQQLMAK GSDKRRGGPP GPPINRGLPL VDDGGWNTVP ISKGSRPIDT SRLTKITKPG SIDSNNQLFA PGGRLSWGKG SSGGSGAKPS DTASEATRPA TLNRFSALQQ TLPAENTDNR RVVQRSSLSR ERGEKAGDRG DRLERSERGG DRGDRLDRAR TPATKRSFSK EVEERSRERP SQPEGLRKAA SLTEDRGRDP VKREATLPPV SPPKAALSVD EVEKKSKAII EEYLHLNDMK EAVQCVQELA SPSLLFIFVR LGIESTLERS TIAREHMGRL LHQLLCAGHL STAQYYQGLY ETLELAEDME IDIPHVWLYL AELITPILQE DGVPMGELFR EITKPLRPMG KATSLLLEIL GLLCKSMGPK KVGMLWREAG LSWREFLAEG QDVGSFVAEK KVEYTLGEES EAPGQRTLAF EELRRQLEKL LKDGGSNQRV FDWIDANLNE QQIASNTLVR ALMTTVCYSA IIFETPLRVD VQVLKVRARL LQKYLCDEQK ELQALYALQA LVVTLEQPAN LLRMFFDALY DEDVVKEDAF YSWESSKDPA EQQGKGVALK SVTAFFNWLR EAEDEESDHN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EIF4G1 (Eukaryotic Translation Initiation Factor 4 Gamma, 1 (EIF4G1))

Alternative Name: Eif4g1 (EIF4G1 Products)

Synonyms: EIF4GI Protein, CG10811 Protein, Dmel\\CG10811 Protein, EIF4G Protein, Eif4G Protein, deIF4G Protein, eIF-4G Protein, eIF4g Protein, p200 Protein, EIF4G1 Protein, wu:fb50a05 Protein, wu:fc60b06 Protein, eif4g Protein, CUCUMOVIRUS MULTIPLICATION 2 Protein, CUM2 Protein, EUKARYOTIC TRANSLATION INITIATION FACTOR 4G Protein, eukaryotic translation initiation factor 4G Protein, DDBDRAFT_0217646 Protein, DDBDRAFT_0217647 Protein, DDBDRAFT_0234257 Protein, DDB_0217646 Protein, DDB_0217647 Protein, DDB_0234257 Protein, NV18824 Protein, EIF-4G1 Protein, EIF4F Protein, P220 Protein, PARK18 Protein, p220 Protein, E030015G23Rik Protein, eIF4GI Protein, eukaryotic translation initiation factor 4 gamma 1 Protein, eukaryotic translation initiation factor 4G Protein, eukaryotic translation initiation factor 4 gamma, 1a Protein, eukaryotic translation initiation factor 4 gamma, 1 S homeolog Protein, eukaryotic initiation factor Protein, eukaryotic translation initiation factor 4 gamma Protein, eukaryotic translation initiation factor 4 gamma, 1 Protein, eukaryotic translation initiation factor 4, gamma 1 Protein, EIF4G1 Protein, eIF4G Protein, eif4g1a Protein, LOC704923 Protein, eif4g1.S Protein, EIF4G Protein, eIF4g Protein, Eif4g Protein, Eif4g1 Protein

Background: Eukaryotic translation initiation factor 4 gamma 1 (eIF-4-gamma 1) (eIF-4G 1) (eIF-4G1),FUNCTION: Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome. Exists in two complexes, either with EIF1 or with EIF4E (mutually exclusive). Together with EIF1, is required for leaky scanning, in particular for avoiding cap-proximal start codon. Together with EIF4E, antagonizes the scanning promoted by EIF1-EIF4G1 and locates the start codon (through a TISU element) without scanning. As a member of the eIF4F complex, required for endoplasmic reticulum stress-induced ATF4 mRNA translation. {ECO:0000250|UniProtKB:Q04637}.

Molecular Weight: 176.1 kDa

UniProt: Q6NZJ6