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KIF11 Protein (AA 1-1052) (His tag)

Crystallography grade KIF11 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3135766
  • Target See all KIF11 Proteins
    KIF11 (Kinesin Family Member 11 (KIF11))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1052
    Origin
    Mouse
    Source
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This KIF11 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MASQPSSLKK KEEKGRNIQV VVRCRPFNLA ERKANAHSVV ECDHARKEVS VRTAGLTDKT SKKTYTFDMV FGASTKQIDV YRSVVCPILD EVIMGYNCTI FAYGQTGTGK TFTMEGERSP NEVYTWEEDP LAGIIPRTLH QIFEKLTDNG TEFSVKVSLL EIYNEELFDL LSPSSDVSER LQMFDDPRNK RGVIIKGLEE ITVHNKDEVY QILEKGAAKR TTAATLMNAY SSRSHSVFSV TIHMKETTID GEELVKIGKL NLVDLAGSEN IGRSGAVDKR AREAGNINQS LLTLGRVITA LVERTPHIPY RESKLTRILQ DSLGGRTRTS IIATISPASF NLEETLSTLE YAHRAKNIMN KPEVNQKLTK KALIKEYTEE IERLKRDLAA AREKNGVYIS EESFRAMNGK VTVQEEQIVE LVEKIAVLEE ELSKATELFM DSKNELDQCK SDLQTKTQEL ETTQKHLQET KLQLVKEEYV SSALERTEKT LHDTASKLLN TVKETTRAVS GLHSKLDRKR AIDEHNAEAQ ESFGKNLNSL FNNMEELIKD GSAKQKAMLD VHKTLFGNLM SSSVSALDTI TTTALESLVS IPENVSARVS QISDMILEEQ SLAAQSKSVL QGLIDELVTD LFTSLKTIVA PSVVSILNIN KQLQHIFRAS STVAEKVEDQ KREIDSFLSI LCNNLHELRE NTVSSLVESQ KLCGDLTEDL KTIKETHSQE LCQLSSSWAE RFCALEKKYE NIQKPLNSIQ ENTELRSTDI INKTTVHSKK ILAESDGLLQ ELRHFNQEGT QLVEESVGHC SSLNSNLETV SQEITQKCGT LNTSTVHFSD QWASCLSKRK EELENLMEFV NGCCKASSSE ITKKVREQSA AVANQHSSFV AQMTSDEESC KAGSLELDKT IKTGLTKLNC FLKQDLKLDI PTGMTPERKK YLYPTTLVRT EPREQLLDQL QKKQPPMMLN SSEASKETSQ DMDEEREALE QCTEELVSPE TTEHPSADCS SSRGLPFFQR KKPHGKDKEN RGLNPVEKYK VEEASDLSIS KSRLPLHTSI NL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Kif11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product KIF11 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    KIF11 (Kinesin Family Member 11 (KIF11))
    Alternative Name
    Kif11 (KIF11 Products)
    Synonyms
    EG5 Protein, HKSP Protein, KNSL1 Protein, MCLMR Protein, TRIP5 Protein, Eg5 Protein, Kif8 Protein, Kifl1 Protein, Knsl1 Protein, F2H17.19 Protein, F2H17_19 Protein, DDBDRAFT_0189194 Protein, DDBDRAFT_0201556 Protein, DDB_0189194 Protein, DDB_0201556 Protein, XLEg5K1 Protein, XlEg5 Protein, cos2 Protein, costal2 Protein, eg5 Protein, hksp Protein, kif11 Protein, kif11-a Protein, kif11-b Protein, kif11b Protein, knsl1 Protein, trip5 Protein, Cos2 Protein, Costal2 Protein, XLEg5K2 Protein, kif11a Protein, kinesin family member 11 Protein, P-loop containing nucleoside triphosphate hydrolases superfamily protein Protein, kinesin family member 11 S homeolog Protein, kinesin family member 11 L homeolog Protein, KIF11 Protein, Kif11 Protein, AT2G36200 Protein, kif11 Protein, kif11.S Protein, kif11.L Protein
    Background
    Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface. {ECO:0000250|UniProtKB:P52732}.
    Molecular Weight
    119.0 kDa Including tag.
    UniProt
    Q6P9P6
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