Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SYNCRIP Protein (AA 2-623) (His tag)

Crystallography grade SYNCRIP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3135918
  • Target See all SYNCRIP Proteins
    SYNCRIP (Synaptotagmin Binding, Cytoplasmic RNA Interacting Protein (SYNCRIP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-623
    Origin
    • 4
    • 1
    Mouse
    Source
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    This SYNCRIP protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    ATEHVNGNGT EEPMDTTSAV IHSENFQTLL DAGLPQKVAE KLDEIYVAGL VAHSDLDERA IEALKEFNED GALAVLQQFK DSDLSHVQNK SAFLCGVMKT YRQREKQGTK VADSSKGPDE AKIKALLERT GYTLDVTTGQ RKYGGPPPDS VYSGQQPSVG TEIFVGKIPR DLFEDELVPL FEKAGPIWDL RLMMDPLTGL NRGYAFVTFC TKEAAQEAVK LYNNHEIRSG KHIGVCISVA NNRLFVGSIP KSKTKEQILE EFSKVTEGLT DVILYHQPDD KKKNRGFCFL EYEDHKTAAQ ARRRLMSGKV KVWGNVGTVE WADPIEDPDP EVMAKVKVLF VRNLANTVTE EILEKSFSQF GKLERVKKLK DYAFIHFDER DGAVKAMEEM NGKDLEGENI EIVFAKPPDQ KRKERKAQRQ AAKNQMYDDY YYYGPPHMPP PTRGRGRGGR GGYGYPPDYY GYEDYYDYYG YDYHNYRGGY EDPYYGYEDF QVGARGRGGR GARGAAPSRG RGAAPPRGRA GYSQRGGPGS ARGVRGARGG AQQQRGRGVR GARGGRGGNV GGKRKADGYN QPDTKRRQTN NQNWGSQPIA QQPLQGGDHS GNYGYKSENQ EFYQDTFGQQ WK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Syncrip Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Grade
    Crystallography grade
    Top Product
    Discover our top product SYNCRIP Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SYNCRIP (Synaptotagmin Binding, Cytoplasmic RNA Interacting Protein (SYNCRIP))
    Alternative Name
    Syncrip (SYNCRIP Products)
    Synonyms
    GRY-RBP Protein, GRYRBP Protein, HNRNPQ Protein, HNRPQ1 Protein, NSAP1 Protein, PP68 Protein, hnRNP-Q Protein, 2610109K23Rik Protein, 4632417O19Rik Protein, Nsap1 Protein, Nsap1l Protein, pp68 Protein, nsap1 Protein, wu:fe02c03 Protein, wu:fe47h09 Protein, SYNCRIP Protein, hnrpq1 Protein, gry-rbp Protein, rp1-3j17.2 Protein, synaptotagmin binding cytoplasmic RNA interacting protein Protein, synaptotagmin binding, cytoplasmic RNA interacting protein Protein, synaptotagmin binding cytoplasmic RNA interacting protein S homeolog Protein, SYNCRIP Protein, Syncrip Protein, syncrip Protein, syncrip.S Protein
    Background
    Heterogeneous nuclear ribonucleoprotein (hnRNP) implicated in mRNA processing mechanisms. Component of the CRD-mediated complex that promotes MYC mRNA stability. Isoform 1 and isoform 2 are associated in vitro with pre-mRNA, splicing intermediates and mature mRNA protein complexes. Isoform 1 binds to apoB mRNA AU-rich sequences (By similarity). Isoform 1 is part of the APOB mRNA editosome complex and may modulate the postranscriptional C to U RNA-editing of the APOB mRNA through either by binding to A1CF (APOBEC1 complementation factor), to APOBEC1 or to RNA itself (By similarity). May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain (By similarity). Interacts in vitro preferentially with poly(A) and poly(U) RNA sequences. Isoform 2 may be involved in cytoplasmic vesicle-based mRNA transport through interaction with synaptotagmins. {ECO:0000250}.
    Molecular Weight
    70.5 kDa Including tag.
    UniProt
    Q7TMK9
You are here:
Support