PLEKHM1 Protein (AA 1-1074) (Strep Tag)

Catalog No. ABIN3135955

Product Details

Target: PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1074

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PLEKHM1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MLSVENGLDP RAAIQVIKKK LVGSVKALQK QHVSLDTVVT SEDGDANTMC SALEAVFIHG LHAKHIRAEA GGKRKKHTHQ KALPQPVFWP LLKAITHRHI VSDLEHLVFI NTDVGRCRAW LRLALNDGLM ECYLKLLLQE PARLCEYYQP TALLRDAEEA EFLLSFLQGL TSLSFELSYK SAILNEWTLT PLSLSGLCPL SELDPLTTSG AELQRKESLD SISHSSGSED IEVQHSGHKI RRNRKLTASS LSLDTASSSQ LSCSLNSDSC LLQENGPKSP DHSEEPMSYD SDLGMANTDD PDRSLQEVLS EFSKAQVNSA PSSGPNQEPD TPMFQTPLSL HSLATSTHLH FEGSEELFPA HKSSGTSSGG HKHQLLPQET PDEKQLGTAQ AGPAQSTSDQ QPSSPVGGAA GQGSGPWKAL EYGRVGPKLV VSSPTSPKGK SWISEDDFCR PPQEPALKSA AGLCTSPVQD TPESRAALHG PFSQGPRKSC SLGALDKACV PSQACGNAQP APAPAPAPAP APAPAPGVTQ DHKNFCVVHR RQMGLSNPFR GLMKLGTVAR RGAMGIWKEF FCELSPLEFR LYLSDEERTC VESCSLLRCE AVGPAHSDGR FELVFSGKKL ALRASSQDEA EDWLDRVREA LQKVRPQQED EWVNIQYPDQ AEDAPEAPPD SLPPYSTLLP EPAGAQGMQL DWTSAQVPEP DAIKESLLYL YADRTWVPYI FSLSLESLKC FRVRNNEKML SDSHGVETIR DILPDTSLGG PAFFKIITAK AVLKLQAKNT EEATHWRDLV RKVLASYLES AEEAVTLGGS LDEKCQEVLK FATRENGFLL QYLVAIPTEK GLDSQGCFCA GCSRQIGFSF VRPKLCAFSG LYYCDFCHQD DASVIPARII HNWDLTKRPV CRQALKFLAQ IRAQPLINLQ LVNASLYEHV ERMHLIGRSR EQLKLLGDYL GLCRSGALKE LCKRLSHRNY LLESPHRFSV ADLQQIAEGV YEGFLKALIE FASQHVYHCD LCTQRGFICQ ICHHQDIIFP FEFDTTVRCA ECRTVFHQSC QAVVRKGCPR CARRRKYQEQ NVVS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PLEKHM1 (Pleckstrin Homology Domain Containing, Family M (With RUN Domain) Member 1 (PLEKHM1))

Alternative Name: Plekhm1 (PLEKHM1 Products)

Synonyms: AP162 Protein, B2 Protein, OPTB6 Protein, BC038943 Protein, D330036J23Rik Protein, pleckstrin homology and RUN domain containing M1 Protein, pleckstrin homology domain containing, family M (with RUN domain) member 1 Protein, PLEKHM1 Protein, Plekhm1 Protein

Background: Pleckstrin homology domain-containing family M member 1 (PH domain-containing family M member 1),FUNCTION: Acts as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Acts as a dual effector of RAB7A and ARL8B that simultaneously binds these GTPases, bringing about clustering and fusion of late endosomes and lysosomes. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Interaction with Arl8b is a crucial factor in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion (PubMed:25498145). Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970). May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7. May have a role in sialyl-lex-mediated transduction of apoptotic signals (By similarity). Involved in bone resorption (PubMed:27777970). {ECO:0000250|UniProtKB:Q9Y4G2, ECO:0000269|PubMed:25498145, ECO:0000269|PubMed:27777970}.

Molecular Weight: 118.5 kDa

UniProt: Q7TSI1