CADPS Protein (AA 1-1355) (Strep Tag)

Catalog No. ABIN3135985

Product Details

Target: CADPS (Ca++-Dependent Secretion Activator (CADPS))

Protein Type: Recombinant

Protein Characteristics: AA 1-1355

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CADPS protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MLDPSSSEEE SDEILEEERG KDVLGSAASG ARLSPSRTSE GSAGSAGMGG SGAGAGVGAG GGGGSGASSG GGAGGLQPSS RAGGGRPSSP SPSVVSEKEK EELERLQKEE EERKKRLQLY VFVMRCIAYP FNAKQPTDMA RRQQKISKQQ LQTVKDRFQA FLNGETQIVA DEAFMNAVQS YYEVFLKSDR VARMVQSGGC SANDSREVFK KHIEKRVRSL PEIDGLSKET VLSSWMAKFD AIYRGEEDPR KQQARMTASA ASELILSKEQ LYEMFQNILG IKKFEHQLLY NACQLDNPDE QAAQIRRELD GRLQMADQIA RERKFPKFVS KEMENMYIEE LKSSVNLLMA NLESMPVSKG GEFKLQKLKR SHNASIIDMG EESENQLSKS DVLLSFSLEV VIMEVQGLKS LAPNRIVYCT MEVEGGEKLQ TDQAEASKPT WGTQGDFSTT HALPAVKVKL FTESTGVLAL EDKELGRVIL HPTPNSPKQS EWHKMTVSKN CPDQDLKIKL AVRMDKPQNM KHSGYLWTIG KNVWKRWKKR FFVLVQVSQY TFAMCSYREK KAEPQELLQL DGYTVDYTDP QPGLEGGRAF FNAVKEGDTV IFASDDEQDR ILWVQAMYRA TGQSHKPVPP TQVQKLNAKG GNVPQLDAPI SQFSGLKDAD RAQKHGMDEF ISSNPCNFDH ASLFEMVQRL TLDHRLNDSY SCLGWFSPGQ VFVLDEYCAR NGVRGCHRHL CYLRDLLERA ENGAMIDPTL LHYSFAFCAS HVHGNRPDGI GTVTVEEKER FEEIKERLRV LLENQITHFR YCFPFGRPEG ALKATLSLLE RVLMKDIVTP VPQEEVKTVI RKCLEQAALV NYSRLSEYAK IEENQKDAEN VGRLITPAKK LEDTIRLAEL VIEVLQQNEE HHAEPHVDKG EAFAWWSDLM VEHAETFLSL FAVDMDAALE VQPPDTWDSF PLFQLLNDFL RTDYNLCNGK FHKHLQDLFA PLVVRYVDLM ESSIAQSIHR GFERESWEPV KSLTSNLPNV NLPNVNLPKV PNLPVNIPLG IPQMPTFSAP SWMAAIYDAD NGSGTSEDLF WKLDALQTFI RDLHWPEEEF GKHLEQRLKL MASDMIESCV KRTRIAFEVK LQKTSRSTDF RVPQSICTMF NVMVDAKAQS TKLCSMEMGQ EHQYHSKIDE LIEETVKEMI TLLVAKFVTI LEGVLAKLSR YDEGTLFSSF LSFTVKAASK YVDVPKPGMD VADAYVTFVR HSQDVLRDKV NEEMYIERLF DQWYNSSMNI ICTWLTDRMD LQLHIYQLKT LIRMVKKTYR DFRLQGVLDS TLNSKTYETI RNRLTVEEAT ASVSEGGGLQ GISMKDSDEE DEEDD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CADPS (Ca++-Dependent Secretion Activator (CADPS))

Alternative Name: Cadps (CADPS Products)

Synonyms: CADPS1 Protein, CAPS Protein, CAPS1 Protein, Caps Protein, Caps1 Protein, Caps2 Protein, AU067781 Protein, mKIAA1121 Protein, CADPS Protein, cadps Protein, MGC122552 Protein, CAPS-1 Protein, caps Protein, caps1 Protein, si:busm1-1i3.1 Protein, si:busm1-6a2.7 Protein, si:dz6a2.7 Protein, calcium dependent secretion activator Protein, Ca2+-dependent secretion activator Protein, Ca2+-dependent activator protein for secretion b Protein, Ca2+ dependent secretion activator Protein, Ca2+ dependent secretion activator L homeolog Protein, Ca2+-dependent activator protein for secretion a Protein, CADPS Protein, Cadps Protein, cadpsb Protein, cadps Protein, cadps.L Protein, cadpsa Protein

Background: Calcium-dependent secretion activator 1 (Calcium-dependent activator protein for secretion 1) (CAPS-1),FUNCTION: Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates catecholamine loading of DCVs. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles by acting as a PtdIns(4,5)P2-binding protein that acts at prefusion step following ATP-dependent priming and participates in DCVs-membrane fusion. However, it may also participate in small clear synaptic vesicles (SVs) exocytosis and it is unclear whether its function is related to Ca(2+) triggering (By similarity). {ECO:0000250, ECO:0000269|PubMed:15820695}.

Molecular Weight: 153.1 kDa

UniProt: Q80TJ1

Pathways: Synaptic Vesicle Exocytosis