CHST2 Protein (AA 76-530) (His tag)

Catalog No. ABIN3136056

Product Details

Target: CHST2 (Carbohydrate (N-Acetylglucosamine-6-O) Sulfotransferase 2 (CHST2))

Protein Type: Recombinant

Protein Characteristics: AA 76-530

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This CHST2 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: YKWHKEPLQQ CNPDGPLGAA VGAAGAGWGR PGSPPAAPPR AHSRMDPRTP YRPPAAGVGA VPAAAAGSAG AAASLGNATR GTRGGGDKRQ LVYVFTTWRS GSSFFGELFN QNPEVFFLYE PVWHVWQKLY PGDAVSLQGA ARDMLSALYR CDLSVFQLYS PAGSGGRNLT TLGIFGAATN KVVCSSPLCP AYRKEVVGLV DDRVCKKCPP QRLARFEEEC RKYRTLVIKG VRVFDVAVLA PLLKDPALDL KVIHLVRDPR AVASSRIRSR HGLIRESLQV VRSRDPRAHR MPFLEAAGHK LGAKKEGMGG PADYHALGAM EVICNSMAKT LQTALQPPDW LQGHYLVVRY EDLVGDPVKT LRRVYDFVGL LVSPEMEQFA LNMTSGSGSS SKPFVVSARN ATQAANAWRT ALTFQQIKQV EEFCYQPMAV LGYERVNSPE EVKDLSKTLL RKPRL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Chst2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CHST2 (Carbohydrate (N-Acetylglucosamine-6-O) Sulfotransferase 2 (CHST2))

Alternative Name: Chst2 (CHST2 Products)

Synonyms: chst2 Protein, wu:fj55f04 Protein, zgc:154070 Protein, CHST2 Protein, C6ST Protein, GST-2 Protein, GST2 Protein, Gn6ST-1 Protein, glcNAc6ST-1 Protein, AI428561 Protein, AW121776 Protein, C130041E03Rik Protein, Chts2 Protein, GlcNAc6ST Protein, Gn6st Protein, carbohydrate sulfotransferase 2 Protein, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2a Protein, carbohydrate sulfotransferase 4 Protein, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 Protein, carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 L homeolog Protein, CHST2 Protein, chst2a Protein, CHST4 Protein, chst2 Protein, chst2.L Protein, Chst2 Protein

Background: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches. Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains. {ECO:0000269|PubMed:15175329, ECO:0000269|PubMed:9712885}.

Molecular Weight: 50.6 kDa Including tag.

UniProt: Q80WV3

Pathways: Glycosaminoglycan Metabolic Process