Symplekin Protein (SYMPK) (AA 1-1284) (His tag)

Catalog No. ABIN3136057

Product Details

Target: Symplekin (SYMPK)

Protein Type: Recombinant

Protein Characteristics: AA 1-1284

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This Symplekin protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: MASSSGDSVT RRSVASQFFT QEEGPSIDGM TTSERVVDLL NQAALITNDS KITVLKQVQE LIINKDPTLL DNFLDEIIAF QADKSIEVRK FVIGFIEEAC KRDIELLLKL IANLNMLLRD ENVNVVKKAI LTMTQLYKVA LQWMVKSRVI SDLQEACWDM VSSMAGEIIL LLDSDNDGIR THAIKFVEGL IVTLSPRMAD SEVPRRQEHD ISLDRIPRDH PYIQYNVLWE EGKAAVEQLL KFMVHPAISS INLTTALGSL ANIARQRPMF MSEVIQAYET LHANLPPTLA KSQVSSVRKN LKLHLLSVLK HPASLEFQAQ ITTLLVDLGT PQAEIARNMP SSKDSRKRPR DDTDSTLKKM KLEPNLGEDD EDKDLEPGPS GTSKASAQIS GQSDTDITAE FLQPLLTPDN VANLVLISMV YLPETMPASF QAIYTPVESA GTEAQIKHLA RLMATQMTAA GLGPGVEQTK QCKEEPKEEK VVKPESVLIK RRLSVQGQAI SVVGSQSTMS PLEEEVPQAK RRPEPIIPVT QPRLAGAGGR KKIFRLSDVL KPLTDAQVEA MKLGAVKRIL RAEKAVACSG AAQVRIKILA SLVTQFDSGF KAEVLSFILE DVRARLDLAF AWLYQEYNAY LAAGTSGTLD KYEDCLICLL SGLQEKPDQK DGIFTKVVLE APLITESALE VIRKYCEDES RAYLGMSTLG DLIFKRPSRQ FQYLHVLLDL SSHEKDRVRS QALLFIKRMY EKEQLREYVE KFALNYLQLL VHPNPPSVLF GADKDTEVAA PWTEETVKQC LYLYLALLPQ NHKLIHELAA VYTEAIADIK RTVLRVIEQP IRGMGMNSPE LLLLVENCPK GAETLVTRCL HSLTDKVPPS PELVKRVRDL YHKRLPDVRF LIPVLNGLEK KEVIQALPKL IKLNPIVVKE VFNRLLGTQH GEGNSALSPL NPGELLIALH NIDSVKCDMK SIIKATNLCF AERNVYTSEV LAVVMQQLME QSPLPMLLMR TVIQSLTMYP RLGGFVMNIL ARLIMKQVWK YPKVWEGFIK CCQRTKPQSF QVILQLPPQQ LGAVFDKCPE LREPLLAHVR SFTPHQQAHI PNSIMTILEA TGKQEPEVKE APSGPLEEDD LEPLALALAP APAPAPAPAP APRPPQDLIG LRLAQEKALK RQLEEEQKQK PTGIGAPAAC VSSTPSVPAA ARAGPTPAEE VMEYREEGPE CETPAIFISM DDDSGLAETT LLDSSLEGPL PKEAAAVGSS SKDERSPQNL SHAVEEALKT SSPETREPES KGNS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sympk Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Symplekin (SYMPK)

Alternative Name: Sympk (SYMPK Products)

Synonyms: SPK Protein, SYM Protein, CG2097 Protein, Dmel\\CG2097 Protein, dSymp Protein, 1500016F02Rik Protein, 4632415H16Rik Protein, AA125406 Protein, AI449890 Protein, Hfn3g Protein, sympk Protein, MGC75595 Protein, SYMPK Protein, T22C5.3 Protein, DKFZp468C0711 Protein, symplekin Protein, Symplekin Protein, symplekin S homeolog Protein, SYMPK Protein, Sym Protein, Sympk Protein, sympk.S Protein, sympk Protein, LOC552752 Protein, AT1G27595 Protein, LOC5580150 Protein, CpipJ_CPIJ011126 Protein, Bm1_50390 Protein, NAEGRDRAFT_80124 Protein, LOAG_00841 Protein

Background: Scaffold protein that functions as a component of a multimolecular complex involved in histone mRNA 3'-end processing. Specific component of the tight junction (TJ) plaque, but might not be an exclusively junctional component. May have a house-keeping rule. Is involved in pre-mRNA polyadenylation. Enhances SSU72 phosphatase activity (By similarity). {ECO:0000250}.

Molecular Weight: 143.2 kDa Including tag.

UniProt: Q80X82