DISC1 Protein (AA 1-852) (Strep Tag)

Catalog No. ABIN3136109

Product Details

Target: DISC1 (Disrupted in Schizophrenia 1 (DISC1))

Protein Type: Recombinant

Protein Characteristics: AA 1-852

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DISC1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MQGGGPRGAP IHSPSHGADS GHGLPPAVAP QRRRLTRRPG YMRSTAGSGI GFLSPAVGMP HPSSAGLTGQ QSQHSQSKAG QCGLDPGSHC QASLVGKPFL KSSLVPAVAS EGHLHPAQRS MRKRPVHFAV HSKNDSRQSE RLTGSFKPGD SGFWQELLSS DSFKSLAPSL DAPWNKGSRG LKTVKPLASP ALNGPADIAS LPGFQDTFTS SFSFIQLSLG AAGERGEAEG CLPSREAEPL HQRPQEMAAE ASSSDRPHGD PRHLWTFSLH AAPGLADLAQ VTRSSSRQSE CGTVSSSSSD TGFSSQDASS AGGRGDQGGG WADAHGWHTL LREWEPMLQD YLLSNRRQLE VTSLILKLQK CQEKVVEDGD YDTAETLRQR LEELEQEKGR LSWALPSQQP ALRSFLGYLA AQIQVALHGA TQRAGSDDPE APLEGQLRTT AQDSLPASIT RRDWLIREKQ RLQKEIEALQ ARMSALEAKE KRLSQELEEQ EVLLRWPGCD LMALVAQMSP GQLQEVSKAL GETLTSANQA PFQVEPPETL RSLRERTKSL NLAVRELTAQ VCSGEKLCSS LRRRLSDLDT RLPALLEAKM LALSGSCFST AKELTEEIWA LSSEREGLEM FLGRLLALSS RNSRRLGIVK EDHLRCRQDL ALQDAAHKTR MKANTVKCME VLEGQLSSCR CPLLGRVWKA DLETCQLLMQ SLQLQEAGSS PHAEDEEQVH STGEAAQTAA LAVPRTPHPE EEKSPLQVLQ EWDTHSALSP HCAAGPWKED SHIVSAEVGE KCEAIGVKLL HLEDQLLGAM YSHDEALFQS LQGELQTVKE TLQAMILQLQ PTKEAGEASA SYPTAGAQET EA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DISC1 (Disrupted in Schizophrenia 1 (DISC1))

Alternative Name: Disc1 (DISC1 Products)

Synonyms: DISC1 Protein, C1orf136 Protein, SCZD9 Protein, disrupted in schizophrenia 1 Protein, disrupted in schizophrenia 1 protein Protein, disc1 Protein, DISC1 Protein, LOC100580606 Protein, Disc1 Protein

Background: Disrupted in schizophrenia 1 homolog,FUNCTION: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis (PubMed:17825401, PubMed:19502360, PubMed:31444471). Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus (PubMed:17825401, PubMed:19502360). Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance (PubMed:19303846). Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation (PubMed:19778506). Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A (PubMed:19778506). Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development (PubMed:19502360). Inhibits ATF4 transcription factor activity in neurons by disrupting ATF4 dimerization and DNA-binding (PubMed:31444471). Plays a role, together with PCNT, in the microtubule network formation (By similarity). {ECO:0000250|UniProtKB:Q9NRI5, ECO:0000269|PubMed:17825401, ECO:0000269|PubMed:19303846, ECO:0000269|PubMed:19502360, ECO:0000269|PubMed:19778506, ECO:0000269|PubMed:31444471}.

Molecular Weight: 92.5 kDa

UniProt: Q811T9

Pathways: Regulation of Cell Size