ERCC6L Protein (AA 1-1240) (Strep Tag)

Catalog No. ABIN3136167

Product Details

Target: ERCC6L (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like (ERCC6L))

Protein Type: Recombinant

Protein Characteristics: AA 1-1240

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ERCC6L protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEASQGLAEV ETLSPQLAES YLRYVQEAKE AAKNGDLEES LKLFNLAKDI FPTKKVMSRI QKLQEALEQL AEEEDDDEFI DVCSSGLLLY RELYEKLFEH QKEGIAFLYS LYKDGRKGGI LADDMGLGKT VQIIAFLSGM FDASLVNHVL LIMPTNLINT WVNEFAKWTP GMRVKTFHGS SKSERTRSLT RIQQRNGVVI TTYQMLLNNW QQLASFNGQA FVWDYVILDE AHKIKSASTK SAVCARAIPA SNRLLLTGTP VQNNLQELWS LFDFACQGSL LGTLKTFKME YEHPIIRARE KDATPGEKAL GLKISENLME IIKPYFLRRT KEEVQTKKAD NPEARLGEKN PAGEAICDMF SLARKNDLIV WIRLLPLQEE IYRKFVSLDH IKELLMETRS PLAELGVLKK LCDHPRLLSA RACRLLNLGT ATFSAQDENE QEDVSNMNSI DHLPDKTLIQ ESGKMIFLMS LLERLQDEGH QTLVFSQSIK ILNIIERLLK NKHFKTLRID GTVTHLWERE KRIQLFQQNK EYSVFLLTTQ VGGVGLTLTA ATRVVIFDPS WNPATDAQAV DRVYRIGQKE NVVVYRLITC GTVEEKIYRR QVFKDSLIRQ TTGEKKNPFR YFTKQELKEL FTVGDLQKSA TQMQLQCLHA AQRRSDEKLD EHIAYLHLLG IAGISDHDLM FTRDLSVKEE LDMLEDSQYI HQRVQKAQFL VESESQNTVQ RQTTGIEETW LKAQEFPSQQ KKKGTEFNKP QPQPSRLLTK PTQVEAISSQ MASITICDQS AESEPQEHSE VHDVTSLQGS HHFNSTSDAG TIASLPQGAE SIGEVSTDSL LSPAKGFAAE NDAMQKKGLQ ASPGQEAPSE NLGSFHYLPR ESSKASLGPN LDLQDSVVLY HRSPTANENQ NLESDVPMIE ISDDLSEPPS ALQGAQAIEA QLELKEDDPL KSPPQYACDF NLFLEDSADT RQNLSSKFLE HVEKEKSLQS PAANSRAKSA LTLSLDSSPK SDEESEVISV KTKSKTRRIL SDDEDEDEED AFKGSHTNSI NISPFPFSSV KQFDASTPQS GSNPSRRFFS PKTPGEVNTS LHSRRSLASR RSLINVVLDD VEDMEERLDN SSEEESEPGL SEENNEEEAL ACTEEQPSGA TLASGNKSSN LTMSEPTSPA PQSSPCAPEP SSSDPMPDPP QDLAVEAGND YESLVARGKE LKECGKIQEA LNCLVKALDI KSADPEVMLM TLSLYKQLNI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ERCC6L (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like (ERCC6L))

Alternative Name: Ercc6l (ERCC6L Products)

Synonyms: PICH Protein, RAD26L Protein, BC004701 Protein, D330021P09Rik Protein, RGD1565734 Protein, fc22h03 Protein, si:ch211-278b8.3 Protein, wu:fc22h03 Protein, ERCC excision repair 6 like, spindle assembly checkpoint helicase Protein, excision repair cross-complementing rodent repair deficiency complementation group 6 like Protein, DNA excision repair protein ERCC-6-like Protein, excision repair cross-complementation group 6-like Protein, ERCC6L Protein, Ercc6l Protein, LOC100373376 Protein, LOC100494766 Protein, LOC100552957 Protein, LOC100560751 Protein, LOC100563247 Protein, ercc6l Protein

Background: DNA excision repair protein ERCC-6-like (EC 3.6.4.12) (ATP-dependent helicase ERCC6-like),FUNCTION: DNA helicase that acts as a tension sensor that associates with catenated DNA which is stretched under tension until it is resolved during anaphase. Functions as ATP-dependent DNA translocase. Can promote Holliday junction branch migration (in vitro). {ECO:0000250|UniProtKB:Q2NKX8}.

Molecular Weight: 138.9 kDa

UniProt: Q8BHK9