CTF18 Protein (AA 1-969) (Strep Tag)

Catalog No. ABIN3136182

Product Details

Target: CTF18 (CHTF18) (CTF18, Chromosome Transmission Fidelity Factor 18 Homolog (CHTF18))

Protein Type: Recombinant

Protein Characteristics: AA 1-969

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CTF18 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEDYEEDLYG VEDDFQNQFA AELEVLAELE GTRDQAPPGT LQTPASRPPL TFEEAIAGGD TVPRPCPAGS PGNVNRNTRK NVRRDQPAPS SPMVKRPRLD VVKKLNFEPD MEELLYPDSP PGDITPPPSP EVFPEMLDAG YSDANADKDL MQTLPSPRNR NPVLRRPPIL EDYINVTSTS GERAFLVLRA DLIGPGVQNP LLDVHWRGCG QLDLLGVPFA SLKEQVDSKR RQQLLEDAQQ LSDTLHSLRS EGEEAVLEGP PAEEPAPGQN TAQHCLWVDE FAPQHYTELL SDDFTNRCLL KWLKLWDLVV FGRERPARKP RPGVETTRVG KEATAPGKWK SHEQALEEML EAELDPSQRP RQKVALLCGP PGLGKTTLAH VVARHAGYCV VEMNASDDRS PEAFRTRIEA ATQMESVLGV GGRPNCLVID EIDGAPTAAI NVLLGILNRK GPQEADQGGT AVAAGGRRRR AEGGLLTRPI ICICNDQFTP SLRQLKQQAL LLHVPPTLPS RLVQRLQEIS LQHGMRSDPG ALVALCEKTD NDIRACINTL QFLYGRGRRE LSVKAVQTTH VGLKDQRKGL FSVWQEVFQL PRTQRRIVGQ DLMLPAHALL LSNGDKGSLT LASQRFYHIL RVTTSAGEHE KVVQGLFDNF LRLRLRDSSL STVCCALDWL AFDDLLEQAA HRGQSFQLLC YLPFVPAAFH VLFASSHVPR ITFPSSQQEA QTRMSQTRNH IQTLVSGMAP TTRSRATPQA LVLDTLCLLL DVLAPKLRPV STQLYSAHEK QQLSCLVGTM LAYSLTYHQE RTPDGQYLYK LEPNVEEVCR FPELPARKPL TYQAKQLIAR EIEMEKMRRA EALAWARSGP QVDQGSSGPA SLWTDSGEKG TRQPAPRNHE QRLEHIMKRA TVQEQPERDF FGRVVIRKVA VPSREVEAPQ KDADEWRMGV AVGRSEVWFR FNEGVSNAVR RSLYIRDLL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CTF18 (CHTF18) (CTF18, Chromosome Transmission Fidelity Factor 18 Homolog (CHTF18))

Alternative Name: Chtf18 (CHTF18 Products)

Synonyms: CHTF18 Protein, zgc:113153 Protein, MGC81266 Protein, 6030457M03Rik Protein, CTF18 Protein, Chl12 Protein, C16orf41 Protein, C321D2.2 Protein, C321D2.3 Protein, C321D2.4 Protein, CHL12 Protein, Ctf18 Protein, RUVBL Protein, chromosome transmission fidelity factor 18 Protein, CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae) Protein, chromosome transmission fidelity factor 18 L homeolog Protein, chromosome transmission fidelity protein 18 homolog Protein, CTF18, chromosome transmission fidelity factor 18 Protein, Chtf18 Protein, CHTF18 Protein, chtf18 Protein, chtf18.L Protein, LOC726742 Protein, LOC100160476 Protein, LOC100433034 Protein, LOC100442428 Protein, LOC100632105 Protein, LOC100642927 Protein

Background: Chromosome transmission fidelity protein 18 homolog,FUNCTION: Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single-stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18-RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. Interacts with and stimulates DNA polymerase POLH. During DNA repair synthesis, involved in loading DNA polymerase POLE at the sites of local damage. {ECO:0000250|UniProtKB:Q8WVB6}.

Molecular Weight: 108.1 kDa

UniProt: Q8BIW9