FBXO38 Protein (AA 1-1194) (Strep Tag)

Catalog No. ABIN3136233

Product Details

Target: FBXO38 (F-Box Protein 38 (FBXO38))

Protein Type: Recombinant

Protein Characteristics: AA 1-1194

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FBXO38 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MGPRKKSAKV CVMDSEVAEE MTADEEKDYM NQLSHEVLCH IFRYLPLQDI MCMECLSRKL KEAVTLYLRV VRVVDLCAGR WWEYMPSGFT DSSFLTLLKK MPDVEQLYGL HPRYLERRRV RGQEAFSIPG VLEALQACPN LVGVETSHLE LVESIWTYMP HVHILGKFRN RNGAFPIPPE NKLKIPIGAK IQTLHLVGVN VPEIPCIPML RHLYMKWVRL TKPQPFKDFL CISLRTFVMR NCAGPTNSLK YVPLVTGLAS ARNLEHLEMV RVPFLGGLIQ HVVEDSWRSG GFRNLHTIVL GACKNALEVD LGYLIITAAR RLHEVRIQPS LTKDGVFSAL KMAELEFPQF ETLHLGYVDE FLLQSRMANA DLVKYGLADV VENPGIITDI GMKAVNEVFS CIKYLAIYNC PHLHNPYNWI SDHSRWMRLV DINLVRCHAL KLDSFGQFVE LLPSLEFISL DQMFREPPKG CARVGLSAGT GIGVSSALVS NQNSNNDNDN NAPNNNANLH DNNHHHPDDS DDDNDFRPDL QAGEAQFAAD ALNEMEDMVQ EDGELVAESG NGMPAHNREV LPVDADEEQA GPSGLQRVVK PTPIADHDSE SDDEEDSLEL QEVWAPKNGT RRYSEREEKT GDSGQSRETA VSGKGKTPLR KRCNNSHQTG QAKPFPLEES SCEKGCQVTS EQIKADMKAA RDVSEKKKSK DVYPSCSSSS SSTAASTAGN ASSPSTASQS PDFARTVTSS GSSEPSPPEV DVSRQCVCSP GGSEDSEAME EGDAESSVCP RCCCLRPQES QRRTGRCSDE ERPSTSRACV VNGADGTRSA FSFRTLPQGG SSGPAHDERT NGSGCGATGE DRRGSSQPES CDVQSNEDYP RRPLTRARSR LSHVPLISES EVAKTKPCHA MKRKRTADKS TSTSDPVIED DHVQVLVLKS KNLVGVTMTN CGITDLVLKD CPKMMFIHAT RCRVLKHLKV ENAPIVNRFD YAQCKKLNMD QVLDQILRMP PERNRIIYLR PMQQVDTLTL EQKLFSGPYP YHICIIHEFS NPPNVRNKVR IRNWMDTIAN INQELIKYEF FLEATRTEED LKKYPKYPWG REIYTLEGVV DGAPYSMISD FPWLRSLRTA EPNSFARYDF EDDEESTIYA PRRKGQLSAD ICMETIGEEI SEMRQMKRGI FQRVVAIFIH YCDVNGEPVE DDYI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FBXO38 (F-Box Protein 38 (FBXO38))

Alternative Name: Fbxo38 (FBXO38 Products)

Synonyms: Fbx38 Protein, MOKA Protein, 6030410I24Rik Protein, AU044865 Protein, AW214031 Protein, Moka Protein, SP329 Protein, MGC131229 Protein, F-box protein 38 Protein, F-box protein 38 L homeolog Protein, FBXO38 Protein, Fbxo38 Protein, fbxo38.L Protein, fbxo38 Protein

Background: F-box only protein 38 (Modulator of KLF7 activity) (MoKA),FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of PDCD1/PD-1, thereby regulating T-cells-mediated immunity (PubMed:30487606). Required for anti-tumor activity of T-cells by promoting the degradation of PDCD1/PD-1, the PDCD1-mediated inhibitory pathway being exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (PubMed:30487606). May indirectly stimulate the activity of transcription factor KLF7, a regulator of neuronal differentiation, without promoting KLF7 ubiquitination (PubMed:14729953, PubMed:16990251). {ECO:0000269|PubMed:14729953, ECO:0000269|PubMed:16990251, ECO:0000269|PubMed:30487606}.

Molecular Weight: 133.9 kDa

UniProt: Q8BMI0