TRERF1 Protein (AA 1-1205) (Strep Tag)

Catalog No. ABIN3136303

Product Details

Target: TRERF1 (Transcriptional Regulating Factor 1 (TRERF1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1205

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TRERF1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MGDQQLYKTN HVGHGGENLF YQQPPLGVHS GLGHSYGNTI SGAGMDAPQA SPISPHFPQD TRDGLGLPIG SKNLGQMDTS RQGGWGSHAG PGNHVQLRSN LANSNMMWGT PTQVEPADGY QYTYSQASEI RTQKLTSGVL HKLDSFTQVF ANQNLRIQVN NMAQVLHTQS AVMDGASDSA LRQLLSQKPV EPSASAIASR YQQVPQQPHP GFTGGLPKPA LPVGQHAPQG HLYYDYQQPL AQMSMQGGQP LQAPQVLSGH MQQLQQHQYY PQPPPQQQQA GLQRISVQEM QQQQQPQQIR PSPPQQQQQL QLQQRQSSLQ IPQYYQPQPM MQHLQEQQQP SMHLQPPSYH RDPHQYTPEQ AHAVQLIQLG SMPQYYYQEP QQAYSHPLYP QSHLSQHQQR EDGQLKTYSS DRQTPAMLSS HGDMGTSDTG VADPASSEMT RVTSTLPHQP LLSPSGIHLN NMGSQHQQPP SPSAMWPQMH LPDGRAQSGS PESSSGQTKG VFGEQFDAKN KLTCSICLKE FKSLPALNGH MRSHGGMRAS PSLKQEEGEK APPPQPQPQP QPQQPLPPPP PPPPPPQLPP EAERLTPMVM PVSVPVKLIP PKPSSQGFTN SVAATPAARD KPASSMSDDE MPVLEIPRKH PPIAAKVEEP LKNLPEKKKF RHRPEPLFIP PPPSSYTPNP TSYSGATLYQ SQLRSPRILG DHLLLDPAHE LPPYTPPPML SPVRQGSGLF SNVLISGHGP GVHPQLPLTP LTPTPRVLLC RSSSIDGSNV TVTPGPGEQT VDVEPRINIG LRFQAEIPEL QDVSALAQDT HRATLVWKPW PELENQALQQ QVENLLNLCC SSALPGGGTN SEFALHSLFE AKGDVMATLE MLLLRKPVRL KCHPLANYHY AGSDKWTSLE RKLFNKALAT YSKDFIFVQK MVKSKTVAQC VEYYYTWKKI MRLGRKHRTR LAEIIDDCMT SEEEEEAEEE EEDPEEDRKS IKEEESEVAK SPEPPPAPAL APTEGPPMQA VGQQPSGNFI CEMPNCGAVF SSRQALNGHA RIHGGTNQVA KTRGAIPSGK QKPGGTQSGY CSVKSSPSHS TTSGETDPTT IFPCKECGKV FFKIKSRNAH MKTHRQQEEQ QRQKAQKAAF AAEMAATIER TTGPVGAPEL LPLDQLSLMK PVKDVDILDD DVVQQLGVMD EAEVVGTDLL LDDQDSVLLQ GDTEL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TRERF1 (Transcriptional Regulating Factor 1 (TRERF1))

Alternative Name: Trerf1 (TRERF1 Products)

Synonyms: BCAR2 Protein, HSA277276 Protein, RAPA Protein, RP1-139D8.5 Protein, TREP132 Protein, TReP-132 Protein, dJ139D8.5 Protein, 9430096I18Rik Protein, AI429294 Protein, B830015H24 Protein, Trep-132 Protein, Trep132 Protein, transcriptional regulating factor 1 Protein, TRERF1 Protein, Trerf1 Protein

Background: Transcriptional-regulating factor 1 (Transcriptional-regulating protein 132) (Zinc finger transcription factor TReP-132),FUNCTION: Binds DNA and activates transcription of CYP11A1. Interaction with CREBBP and EP300 results in a synergistic transcriptional activation of CYP11A1. {ECO:0000250|UniProtKB:Q96PN7}.

Molecular Weight: 132.4 kDa

UniProt: Q8BXJ2

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Chromatin Binding