CPNE1 Protein (AA 1-536) (Strep Tag)

Catalog No. ABIN3136354

Product Details

Target: CPNE1 (Copine I (CPNE1))

Protein Type: Recombinant

Protein Characteristics: AA 1-536

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CPNE1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAHCVTLVQL SVSCEHLIDK DIGSKSDPLC VLLQDVGGAW AELCRTERVR NCSSPEFSKT LQIEYHFETV QKLRFGIYDI DNKTPELGDD DFLGGAECSL GQIVSSQTLT LPLMLKPGKP AGRGTITVSA QELKDSRVVT MEVEARNLDK KDFLGKSDPF LEFFRQGDGK WQLAYRTEVV KNNLNPTWKR FSVSLQHFCG GDLSTPIQVR CSDYDSDGSH DLIGTFHTTL AQLQAVPAEF ECVHPEKQQR KKNYRNSGTV RVKTCRVETE YSFLDYVMGG CQINFTVGVD FTGSNGDPSS PDSLHYLSPT GVNEYLTALW SVGSVVQDYD SDKLFPAFGF GAQVPPDWQV SHEFALNFNP SNPYCAGIQG IVDAYRQALP QVRLYGPTNF APIINHVARF AAQAAQQRSA SQYFVLLLLT DGAVTDVEAT CKAVVDASKL PMSVIIVGVG GADFEVMEQL DADGGPLRTR SGEAAARDIV QFVPYRRFQN APRETLAQTV LAEVPTQMVS YFRAQGWAPL KAPPTPGKGP AQAPQA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CPNE1 (Copine I (CPNE1))

Alternative Name: Cpne1 (CPNE1 Products)

Synonyms: CPNE1 Protein, MGC108079 Protein, COPN1 Protein, CPN1 Protein, 1810028N16Rik Protein, mKIAA4108 Protein, cpne3 Protein, cpne3l Protein, wu:fb69d07 Protein, wu:fc45a04 Protein, zgc:55873 Protein, copine I Protein, copine 1 Protein, RNA-binding protein 12 Protein, copine-1 Protein, copine I L homeolog Protein, CPNE1 Protein, cpne1 Protein, CPNE1b Protein, LOC100032499 Protein, LOC100484845 Protein, EDI_033120 Protein, LOC100281056 Protein, cpne1.L Protein, Cpne1 Protein

Background: Copine-1 (Copine I),FUNCTION: Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner. Exhibits calcium-dependent phospholipid binding properties. Plays a role in neuronal progenitor cell differentiation, induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner. May recruit target proteins to the cell membrane in a calcium-dependent manner. May function in membrane trafficking. Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit. Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL. {ECO:0000250|UniProtKB:Q99829}.

Molecular Weight: 58.9 kDa

UniProt: Q8C166