TAF2 Protein (AA 1-1104) (Strep Tag)

Catalog No. ABIN3136355

Product Details

Target: TAF2 (TAF2 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 150kDa (TAF2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1104

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TAF2 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MNRKKGDKGF ESPRPYKLTH QVVCINNINF QRKSVVGFVE LTIFPTVANL NRIKLNSKQC RIYRVRINDL EAAFIYNDPT LEVCHSESKQ RNLNYFSNAY AAAVSAVDPD AGNGELCIKV PSELWKHVDE LKVLKIHINF SLDQPKGGLH FVVPSVEGSM AERGAHVFSC GYQNSTRFWF PCVDSYSELC TWKLEFTVDA AMVAVSNGDL VETVYTHDMR KKTFHYMLTI PTAASNISLA IGPFEILVDP YMHEVTHFCL PQLLPLLKHT TSYIHEVFEF YEEILTCRYP YSCFKTVFID EAYVEVAAYA SMSIFSTNLL HSAMIIDETP LTRRCLAQAL AQQFFGCFIS RMSWSDEWVL KGISGYIYGL WMKKTFGVNE YHHWIKEELD KIVAYELKTG GVLLHPIFGG GKEKDNPASH LHFSIKHPHT LSWEYYTMFQ CKAHLVMRLI ENRISMEFML QVFNKLLSLA STASSQKFQS HMWSQMLVST YGFLKSISNV SGKDIQPLIK QWLDQSGVVK FYGSFAFNRK RNVLELEIKQ DYTSPGTQKY VGPLKVTVQE LDGSFNHTLQ IEENSLKHDI PCHSKSRRNK KKKIPLMNGE EVDMDLSAME ADSPLLWIRI DPDMSVLRKV EFEQADFMWQ YELRYERDVV AQQESILALE KFPTPASRLA LTDILEQEQC FYRVRMSACF CLAKIANSMV STWTGPPAMK SLFTRMFCCK TCPNIVKTNN FMSFQSYFLQ KTMPVAMALL RDVHNLCPKE VLTFILDLIK YNDNRKNKFS DNYYRAEMID ALANSVTPAV SVNNEVRTLD NLNPDVRLIL EEITRFLNME KLLPSYRHTI TVSCLRAIRV LQKNGHVPSD ASLFKSYAEY GHFVDIRIAA LEAVVDYTKV DRSYEELQWL LNMIQTDPVP YVRHKILNML TKNPPFTKNM ESPLCNEALV DQLWKLMNSG TAHDWRLRCG AVDLYFTLFG LSRPSCLPLP ELGLVLNLKE KKAVLNPTII PEAGVGNQFS SSQDEEEVDM DTVHDSQAFI SHHLNMLERP STPGLSKYRP HHHHHHHEHK KKKKKHKHKH KHKHKHDSKD KDREPFAFSS PASGRSVRSP SLSD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TAF2 (TAF2 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 150kDa (TAF2))

Alternative Name: Taf2 (TAF2 Products)

Synonyms: CG6711 Protein, Dmel\\CG6711 Protein, TAF Protein, TAF150 Protein, TAF2 Protein, TAFII150 Protein, TAF[II]150 Protein, TAF[[II]] Protein, TAF[[II]]150 Protein, TFIID Protein, Taf150 Protein, TafII150 Protein, Taf[[II]]150 Protein, dTAF II 150 Protein, dTAF150 Protein, dTAFII150 Protein, dTAF[[II]]150 Protein, TAFII-150 Protein, fc30f02 Protein, wu:fc30f02 Protein, 150kDa Protein, 4732460C16Rik Protein, AI425886 Protein, CIF150 Protein, TAF2B Protein, TAFIIB Protein, TBP-associated factor 2 Protein, TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor Protein, TATA-box binding protein associated factor 2 Protein, TATA-box binding protein associated factor 2 S homeolog Protein, Taf2 Protein, taf2 Protein, taf2.S Protein, TAF2 Protein

Background: Transcription initiation factor TFIID subunit 2 (TBP-associated factor 150 kDa) (Transcription initiation factor TFIID 150 kDa subunit) (TAF(II)150) (TAFII-150) (TAFII150),FUNCTION: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF2 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF1. {ECO:0000250|UniProtKB:Q6P1X5}.

Molecular Weight: 127.0 kDa

UniProt: Q8C176