CNOT3 Protein (AA 1-751) (Strep Tag)

Catalog No. ABIN3136529

Product Details

Target: CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))

Protein Type: Recombinant

Protein Characteristics: AA 1-751

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CNOT3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD QIKTWVASNE IKDKRQLIEN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP AKNGSKPVHS NQHPQSPAVP PTYPSGPPPT TSALSSTPGN NGASTPAAPT SALGPKASPA PSHNSGTPAP YAQAVAPPNA SGPSNAQPRP PSAQPSGGSG GGSGGSSSNS NSGTGGGAGK QNGATSYSSV VADSPAEVTL SSSGGSSASS QALGPTSGPH NPAPSTSKES STAAPSGAGN VASGSGNNSG GPSLLVPLPV NPPSSPTPSF SEAKAAGTLL NGPPQFSTTP EIKAPEPLSS LKSMAERAAI SSGIEDPVPT LHLTDRDIIL SSTSAPPTSS QPPLQLSEVN IPLSLGVCPL GPVSLTKEQL YQQAMEEAAW HHMPHPSDSE RIRQYLPRNP CPTPPYHHQM PPPHSDTVEF YQRLSTETLF FIFYYLEGTK AQYLAAKALK KQSWRFHTKY MMWFQRHEEP KTITDEFEQG TYIYFDYEKW GQRKKEGFTF EYRYLEDRDL Q
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))

Alternative Name: Cnot3 (CNOT3 Products)

Synonyms: LENG2 Protein, NOT3 Protein, NOT3H Protein, A930039N10Rik Protein, cnot3 Protein, zgc:55934 Protein, wu:fi20g07 Protein, fb73b06 Protein, fc17h07 Protein, wu:fb73b06 Protein, wu:fc17h07 Protein, zgc:92813 Protein, CNOT3 Protein, Afu2g02070 Protein, AO090011000479 Protein, CG8426 Protein, Dmel\\CG8426 Protein, NOT3.5 Protein, NOT3/5 Protein, Not3 Protein, not3 Protein, not3/5 Protein, leng2 Protein, not3h Protein, CCR4-NOT transcription complex subunit 3 Protein, CCR4-NOT transcription complex, subunit 3 Protein, CCR4-NOT transcription complex, subunit 3b Protein, CCR4-NOT transcription complex, subunit 3a Protein, CCR4-NOT transcription complex subunit 3 S homeolog Protein, CNOT3 Protein, Cnot3 Protein, cnot3b Protein, cnot3a Protein, AFUA_2G02070 Protein, NFIA_034440 Protein, ACLA_092760 Protein, PMAA_089540 Protein, AFLA_043060 Protein, TSTA_125290 Protein, ANI_1_738064 Protein, AOR_1_812054 Protein, Not3 Protein, cnot3 Protein, cnot3.S Protein

Background: CCR4-NOT transcription complex subunit 3 (CCR4-associated factor 3),FUNCTION: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation, may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation, the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity, prevents their differentiation towards extraembryonic trophectoderm lineages. {ECO:0000269|PubMed:21897366, ECO:0000269|PubMed:22367759}.

Molecular Weight: 81.9 kDa

UniProt: Q8K0V4

Pathways: Stem Cell Maintenance