NCAPD2 Protein (AA 1-1392) (Strep Tag)

Catalog No. ABIN3136568

Product Details

Target: NCAPD2 (Non-SMC Condensin I Complex, Subunit D2 (NCAPD2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1392

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NCAPD2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSPHNFEFHL PLSPEELLKS GGVNQYVVRE VLPVKHLSSQ LRAFQSAFRA QGPLAILEHF DTVYSILHHF RSIEPGLKED TLEFLKKVVS RHSQELSSIL DDAALSGSDR SAHLNALKMN CYALIRLLES FENMTSQTSL IDLDIGGKGK RARAKATLGF DWEEERQPVL QLLTQLLQLD IRHLWNHSAI EEEFVSLVTG CCYRLLENPT ISHQKNRSTK EAIAHLLGVA LVRYNHMLSA TVKIIQMLQH FEHLPPVLVT AVSLWATDYG MKSIVGEIVR EIGQKCPQEL SRDTAGAKGF AAFLTELAER IPAVLMANMC ILLDHLDGEN YMMRNAVLAA IAEMVLQVLN GDQLEESARE TRDQFLDILQ AHGHDVNSFV RSRVLQLFAR IVQQKALPLT RFQAVVALAV GRLADKSVLV CKNAIQLLAS FLANNPFSCK LSDIDLAGPL QKEIQKLQEM RAQRRSAAAT AALDPEEEWD AMLPELKSTL QQLLKLPQEE GDHQIADAET AEEVKGRIRQ LLAKASYKQA IVLTREATSH FQESEPFSHT EPEENSFLNL LGLIFKGPEA STQDSHGDTD PGLTGSKDSP SVPEPEGSQS NDELVKQEML VQYLQDAYGF SQKITEAIGI ISKMMYENTT TVVQEVIEFF VMVFQFGVPQ ALFGVRRMLP LIWSKEPGVR EAVLNAYRQL YLNPKGDSAR AKAQTLIHNL SLLLVDASVG TIQCLEEILC EFVQKDEVKP AVIQLLWERA TEKVPSSPLE RCSSVMLLGM MARGKPEIVG SNLDALVRVG LDEKSPQDYR LAQQVCLAIA NISDRRKPSL GERHPPFRLP QEHRLFERLQ DMVTKGFAHP DPLWIPFKEV AVTLTYQLAE SPDVLCAQML QGCAKQVLEK LEKNATEADP KETAPRLPTF LLMNLLSLAG DVALQQLVHL EQAVSGELGR RRVLREEQEH RAKEPKEKTA SSETTMEEEL GLVGGATADD TEAELIRSIC EKELLDGNQV LAAFVPLLLK VCNNPGLYSN PELCAAASLA LGKFCMISAP FCDSQLRLLF TMLEKSSLPT VRSNLMVATG DLAIRFPNLV DPWTPHLYAR LRDPAQQVRK TAGLVMTHLI LKDMVKVKGQ VSEMAVLLID PVPQIAALAK NFFNELSHKG NAIYNLLPDI ISRLSDPEGG VEEEPFHTIM KQLLSYITKD KQTESLVEKL CQRFRTARTE RQYRDLAYCM SQLPLTERGL QKMLDNFECF GDKLLDESVF SAFLSIVGKL RRGAKPEGKA IIDEFEQKLR ACHTRGMDGI EEFETGQGGS QRALSAKKPS AVSRLQPLTS VDSDNDFVTP KPRRTKPGRP QTQQRKKSQR KAKVVFLSDE SSEDELSAEM TEEETPKRTT PIRRASGRRH RS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NCAPD2 (Non-SMC Condensin I Complex, Subunit D2 (NCAPD2))

Alternative Name: Ncapd2 (NCAPD2 Products)

Synonyms: CAP-D2 Protein, CNAP1 Protein, hCAP-D2 Protein, 2810406C15Rik Protein, 2810465G24Rik Protein, mKIAA0159 Protein, RGD1562596 Protein, im:6902697 Protein, si:dkey-175g20.1 Protein, wu:fc13f08 Protein, wu:fx06e10 Protein, non-SMC condensin I complex subunit D2 Protein, non-SMC condensin I complex, subunit D2 Protein, non-SMC condensin I complex subunit D2 S homeolog Protein, condensin complex subunit 1 Protein, NCAPD2 Protein, Ncapd2 Protein, ncapd2.S Protein, CIMG_02087 Protein, BDBG_08990 Protein, MCYG_08389 Protein, VDBG_09812 Protein, MGYG_05811 Protein, ncapd2 Protein

Background: Condensin complex subunit 1 (Chromosome condensation-related SMC-associated protein 1) (Chromosome-associated protein D2) (mCAP-D2) (Non-SMC condensin I complex subunit D2) (XCAP-D2 homolog),FUNCTION: Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. May target the condensin complex to DNA via its C-terminal domain. May promote the resolution of double-strand DNA catenanes (intertwines) between sister chromatids. Condensin-mediated compaction likely increases tension in catenated sister chromatids, providing directionality for type II topoisomerase-mediated strand exchanges toward chromatid decatenation. Required for decatenation of non-centromeric ultrafine DNA bridges during anaphase. Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size. {ECO:0000250|UniProtKB:Q15021}.

Molecular Weight: 155.7 kDa

UniProt: Q8K2Z4