PCGF1 Protein (AA 2-259) (His tag)

Catalog No. ABIN3136627

Product Details

Target: PCGF1 (Polycomb Group Ring Finger 1 (PCGF1))

Protein Type: Recombinant

Protein Characteristics: AA 2-259

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This PCGF1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ASPQGGQIAI AMRLRNQLQS VYKMDPLRNE EEVRVKIKDL NEHIVCCLCA GYFVDATTIT ECLHTFCKSC IVKYLQTSKY CPMCNIKIHE TQPLLNLKLD RVMQDIVYKL VPGLQDSEEK RIREFYQSRG LDRVSQPSGE EPALSNLGLP FSSFDHSKAH YYRYDEQLSL CLERLSSGKD KNKNVLQNKY VRCSVRAEVR HLRRVLCHRL MLNPQHVQLL FDNEVLPDHM TMKQLWLSRW FGKPSPLLLQ YSVKEKRR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Pcgf1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PCGF1 (Polycomb Group Ring Finger 1 (PCGF1))

Alternative Name: Pcgf1 (PCGF1 Products)

Synonyms: PCGF1 Protein, 2010002K04Rik Protein, NSPC1 Protein, RNF3A-2 Protein, RNF68 Protein, AU024121 Protein, Nspc1 Protein, nspc1 Protein, si:zc207o21.2 Protein, polycomb group ring finger 1 Protein, polycomb group ring finger 1 L homeolog Protein, PCGF1 Protein, pcgf1 Protein, Pcgf1 Protein, pcgf1.L Protein

Background: Component of the Polycomb group (PcG) multiprotein BCOR complex, a complex required to maintain the transcriptionally repressive state of some genes, such as BCL6 and the cyclin-dependent kinase inhibitor, CDKN1A. Transcriptional repressor that may be targeted to the DNA by BCL6, this transcription repressor activity may be related to PKC signaling pathway. Represses CDKN1A expression by binding to its promoter, and this repression is dependent on the retinoic acid response element (RARE element). Promotes cell cycle progression and enhances cell proliferation as well. May have a positive role in tumor cell growth by down-regulating CDKN1A. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Regulates the expression of DPPA4 and NANOG in the NT2 embryonic carcinoma cells (By similarity). {ECO:0000250|UniProtKB:Q9BSM1}.

Molecular Weight: 31.1 kDa Including tag.

UniProt: Q8R023