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Nedd4 Family Interacting Protein 1 (NDFIP1) (AA 2-221) protein (rho-1D4 tag)

Details for Product No. ABIN3136636, Supplier: Log in to see
Protein Name
  • 0610010M22Rik
  • MGC108163
  • n4wbp5
  • N4wbp5
  • N4WBP5
  • ndfip1
  • NDFIP1
  • wu:fc17e11
  • zgc:55546
Protein Characteristics
AA 2-221
2
1
1
1
Origin
Mouse (Murine)
4
1
Source
Insect Cells
3
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This NDFIP1 protein is labelled with rho-1D4 tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
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Sequence ALALAALAAV EPACGSGYQQ LQNEEEPGEP EQTAGDAPPP YSSITAESAA YFDYKDESGF PKPPSYNVAT TLPSYDEAER TKTEATIPLV PGRDEDFVGR DDFDDTDQLR IGNDGIFMLT FFMAFLFNWI GFFLSFCLTT SAAGRYGAIS GFGLSLIKWI LIVRFSTYFP GYFDGQYWLW WVFLVLGFLL FLRGFINYAK VRKMPETFSN LPRTRVLFIY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Ndfip1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Purification Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin-free.
Grade Crystallography grade
Background Activates HECT domain-containing several E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (PubMed:11748237, PubMed:17137798). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity (By similarity). Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate (By similarity). Enhances the ubiquitination of BRAT1 mediated by E3 ubiquitin-protein ligases: NEDD4, NEDD4L and ITCH, and is required for the nuclear localization of ubiquitinated BRAT1 (PubMed:25631046). {ECO:0000250|UniProtKB:Q9BT67, ECO:0000269|PubMed:11748237, ECO:0000269|PubMed:17137798, ECO:0000269|PubMed:25631046}.
Molecular Weight 26.0 kDa Including tag.
UniProt Q8R0W6
Research Area Proteolysis / Ubiquitin, Chromatin and Nuclear Signaling, Cell Cycle
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
 image for Nedd4 Family Interacting Protein 1 (NDFIP1) (AA 2-221) protein (rho-1D4 tag) (ABIN3136636) „Crystallography Grade“ protein due to multi-step, protein-specific purification process