NUP85 Protein (AA 1-656) (Strep Tag)

Catalog No. ABIN3136666

Product Details

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Protein Type: Recombinant

Protein Characteristics: AA 1-656

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NUP85 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MEELDCEPAV TWIPGVNSKK KQMCFDWGPG EMLLCETSFN QTGKSEKVPS CPFIYIIRKD VDVYSQILRK LFNESHGIFV GLQKIEEELS GKSRKAQLVR VSKNYRSVIR ACMEEMHQVA IAAKDPASGR QFSSQVSILS AMELIWNLCE ILFIEVAPAG PLLLHLLDWV RLHVCEVDSL SADVLGGDNP SKHENFWDLV TVLVLQGRLD EARQMLAKEA DANPSCAGMC RVLGDLMRTM PILSPGNTQT LTELELKWQH WREECERHLQ DNTFAANPRL ESLCKIMLGD EAALLEQKEL LSNWYHFLVT RLLYSNPTVK PIDLHFYAQS SLDMFLGGES SPEPLDNILM AAFEFDIHQV IKECSIALSN WWFVAHLTDL LDHCRLLQSH NLYFGSNMRE FLLLEYASGL FAHHSLWQLG VDYFDYCPEL GRVSLELHIE RIPLNTEQKA LKVLRICEQR QMTEQVKSIC KILAMKAVRN NRLGSALSWS IRAKDAAFAT LVSDRFLRDY CERGCFSDLD LIDNLGSAMM LSDRLTFLGK YREFHRLYGE KRFGDAASLL LSLMTSQIAP RSFWMTLLTD ALPLLEQKQV IFSAEQTYEL MRCLEDLASG RPECGEPDAQ RLQDDDIETT KVEMLRLALA RNLARAIIRE GSLEGS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Alternative Name: Nup85 (NUP85 Products)

Synonyms: Pcnt1 Protein, frount Protein, pcnt1 Protein, zgc:100943 Protein, Nup75 Protein, nucleoporin 85kDa L homeolog Protein, nucleoporin 85kDa Protein, nucleoporin 85 Protein, nup85.L Protein, nup85 Protein, NUP85 Protein, Nup85 Protein

Background: Nuclear pore complex protein Nup85 (85 kDa nucleoporin) (FROUNT) (Nucleoporin Nup85) (Pericentrin-1),FUNCTION: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade. Involved in nephrogenesis. {ECO:0000250|UniProtKB:Q9BW27}.

Molecular Weight: 74.8 kDa

UniProt: Q8R480