SNX9 Protein (AA 1-595) (Strep Tag)

Catalog No. ABIN3136781

Product Details

Target: SNX9 (Sorting Nexin 9 (SNX9))

Protein Type: Recombinant

Protein Characteristics: AA 1-595

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SNX9 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MATKARVMYD FAAEPGNNEL TVTEGEIITV TNPNVGGGWL EGKNNKGEQG LVPTDYVEIL PNDGKDPFSC GNSVADQAFL DSLTASTAQT NSSSANSNNQ VGGGNDPWTA WNAPKPGNWD SSDAWGSRTD GTSAQRNSSA NNWDTGFGHP QAYQGPATGD DDEWDEDWDD PKSSSPYFKD SEPAEAGGIQ RGNSRAGASS MKLPLNKFPG FAKPGMEQYL LAKQLAKPKE KIAIIVGDYG PMWVYPTSTF DCVVADPRKG SKMYGLKSYI EYQLTPTNTN RSVNHRYKHF DWLYERLLVK FGSAIPIPSL PDKQVTGRFE EEFIKMRMER LQAWMTRMCR HPVVSESEVF QQFLNFRDEK EWKTGKRKAE KDELVGVMIF STMEPEAPDL DLIEIEQKCD AVGKFTKAMD DGVKELLTVG QEHWKRCTGP LPKEYQKIGK ALQSLAAVFS SSGYQGETDL NDAITEAGKT YEEIASLVAE QPKKDLHFLM ECNHEYKGFL GCFPDIIGAH KGAIEKVKES DKLVATSKIT PQDKQTMVKR VGTMSYALQA EMNHFHSNRI YDYNSVIRLY LEQQVQFYET IAEKLRQALS RFPVM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SNX9 (Sorting Nexin 9 (SNX9))

Alternative Name: Snx9 (SNX9 Products)

Synonyms: si:bz36d5.4 Protein, snx9 Protein, zgc:113455 Protein, MGC78823 Protein, SNX9 Protein, SDP1 Protein, SH3PX1 Protein, SH3PXD3A Protein, WISP Protein, 2700073N08Rik Protein, sorting nexin 9a Protein, sorting nexin 9 Protein, sorting nexin 9 L homeolog Protein, snx9a Protein, SNX9 Protein, snx9.L Protein, snx9 Protein, Snx9 Protein

Background: Sorting nexin-9,FUNCTION: Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton (PubMed:23437151). Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate (By similarity). {ECO:0000250, ECO:0000269|PubMed:23437151}.

Molecular Weight: 66.5 kDa

UniProt: Q91VH2