CDSE1 Protein (AA 1-798) (Strep Tag)

Catalog No. ABIN3136793

Product Details

Target: CDSE1 (Cold Shock Domain Containing E1, RNA-Binding (CDSE1))

Protein Type: Recombinant

Protein Characteristics: AA 1-798

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CDSE1 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MSFDPNLLHN NGHNGYPNGT SAALRETGVI EKLLTSYGFI QCSERQARLF FHCSQYNGNL QDLKVGDDVE FEVSSDRRTG KPIAIKLVKI KPEIHPEERM NGQVVCAVPH NLESKSPAAP GQSPTGSVCY ERNGEVFYLT YTSEDVEGNV QLETGDKINF VIDNNKHTGA VSARNIMLLK KKQARCQGVV CAMKEAFGFI ERGDVVKEIF FHYSEFKGDL ETLQPGDDVE FTIKDRNGKE VATDVRLLPQ GTVIFEDISI EHFEGTVTKV IPKVPSKNQN DPLPGRIKVD FVIPKELPFG DKDTKSKVTL LEGDHVRFNI STDRRDKLER ATNIEVLSNT FQFTNEAREM GVIAAMRDGF GFIKCVDRDA RMFFHFSEIL DGNQLHIADE VEFTVVPDML SAQRNHAIRI KKLPKGTVSF HSHSDHRFLG TVEKEATFSN PKTTSPNKGK DKEAEDGIIA YDDCGVKLTI AFQAKDVEGS TSPQIGDKVE FSISDKQRPG QQIATCVRLL GRNSNSKRLL GYVATLKDNF GFIETANHDK EIFFHYSEFS GDVDSLELGD MVEYSLSKGK GNKVSAEKVN KAHSVNGITE EANPTIYSGK VIRPLRGVDP TQIEYQGMIE IVEEGDMKGE VYPFGIVGMA NKGDCLQKGE SVKFQLCVLG QNAQTMAYNI TPLRRATVEC VKDQFGFINY EVGDSKKLFF HVKEVQDGVE LQAGDEVEFS VILNQRTGKC SACNVWRVCE GPKAVAAPRP DRLVNRLKNI TLDDASAPRL MVLRQPRGPD NSMGFGAERK IRQAGVID
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CDSE1 (Cold Shock Domain Containing E1, RNA-Binding (CDSE1))

Alternative Name: Csde1 (CDSE1 Products)

Synonyms: unr Protein, d1s155e Protein, MGC69452 Protein, CSDE1 Protein, DKFZp459E136 Protein, DKFZp459H081 Protein, DKFZp469D1619 Protein, MGC114719 Protein, wu:fa97c10 Protein, AA960392 Protein, BC016898 Protein, D3Jfr1 Protein, mKIAA0885 Protein, Unr Protein, D1S155E Protein, RP5-1000E10.3 Protein, UNR Protein, cold shock domain containing E1 Protein, cold shock domain containing E1 L homeolog Protein, cold shock domain containing E1, RNA-binding Protein, cold shock domain containing E1, RNA binding Protein, csde1 Protein, CSDE1 Protein, csde1.L Protein, Csde1 Protein

Background: Cold shock domain-containing protein E1,FUNCTION: RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules. {ECO:0000250|UniProtKB:O75534}.

Molecular Weight: 88.8 kDa

UniProt: Q91W50

Pathways: SARS-CoV-2 Protein Interactome