SMC6 Protein (AA 1-1097) (Strep Tag)

Catalog No. ABIN3136902

Product Details

Target: SMC6 (SMC6 Structural Maintenance of Chromosomes 6-Like 1 (SMC6))

Protein Type: Recombinant

Protein Characteristics: AA 1-1097

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SMC6 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAKRKEENFC SPENAKRPRQ EELEDFDKDG DEDECTISFT NGTSTLTAAE VGIIESIQLR NFMCHSMLGP FKFGSNVNFV VGNNGSGKSA VLTALIVGLG GKAVATNRGS SLKGFVKAGQ NSADISITLR NRGDDAFRAN VYGDSIVVQQ HISVDGSRSY KLKSEKGTVV STRKEELIAI LDHFNIQVDN PVSVLTQEMS KQFLQSKNEG DKYKFFMKAT QLEQMKEDYS YIMETKERTK EQINQGEERL TELKRQCLEK EERFQNIAGL STMKTNLEYL KHEMAWAVVN EIEKQLNAIR DNIKIGEERA AKLDRKMEEQ QVRLNDAEKK YKDIQDKLEK ISEETNARAP ECMALKTDVI ARTRAFNDAE VLYNRSLNEY KALKKDGEQL CKRIEELKKS TDQSLEPERL ERQKRICWLK EKVKALQDQE HTVNQEAEQF EQAIEKDKQE HGRVRKEDIE VRHALNYNQR QLKELKDSKT DRLKRFGPHV PALLEAIDDA YRRRQFTHKP IGPLGACIHL RDPELALAIE SCLKGLLQAY CCHNHADERV LQSLMKKFYP PGTSRPQIIV SEFRDEVYDV RLRAAYHPEF PTVLTALEID NAVVANSLID MRSIETVLLI KNNSVARAVM QSQKPPKNCR EAFTADGDQV FAGRYYSSES TRPKFLSRDV DSEISDLETE IENKKGHIIT LQQRLSALEK DIKRNEELLK RCQLHYKEIK MKIRKNISEI RELENIEEHQ SVDIATLEDE AEENKIKMQM VEKNMEQQKE NMENLKSLKI EAENKYDTIK LKINQLSELA DPLKDELNLA DSEVDSQKRG KQHYEDKQKE HLDTLNKKRR ELDMKEKELQ EKMSQARQIC PERIEVKKSA SILDKEINRL RQKIQAEHAS HGDREEIMKQ YQEARETYLD LDNKVRTLRR FIKLLEEIMT HRYKTYQQFR RCLTLRCKLY FDNLLSQRAY CGKMNFDHKN ETLSITVQPG EGNKASFNDM RALSGGERSF STVCFILSLW SIAESPFRCL DEFDVYMDMV NRRIAMDMIL KMADSQRFRQ FILLTPQSMS SLPSSKLIRI LRMSDPERGQ TTLPFRPVTQ EEDDSAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SMC6 (SMC6 Structural Maintenance of Chromosomes 6-Like 1 (SMC6))

Alternative Name: Smc6 (SMC6 Products)

Synonyms: SMC6L1 Protein, SMC-6 Protein, hSMC6 Protein, 2810489L22Rik Protein, 3830418C19Rik Protein, AA990493 Protein, AU018782 Protein, AW742439 Protein, Smc6l1 Protein, mKIAA4103 Protein, structural maintenance of chromosomes 6 Protein, SMC6 Protein, smc6 Protein, Smc6 Protein

Background: Structural maintenance of chromosomes protein 6 (SMC protein 6) (SMC-6) (mSMC6),FUNCTION: Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components (By similarity). {ECO:0000250}.

Molecular Weight: 127.2 kDa

UniProt: Q924W5