ST5 Protein (AA 1-1134) (Strep Tag)

Catalog No. ABIN3136904

Product Details

Target: ST5 (Suppression of Tumorigenicity 5 (ST5))

Protein Type: Recombinant

Protein Characteristics: AA 1-1134

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ST5 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MTMTANKNSS ITHGTGGTKA PRETLSRSQS VSPPPVLYPP RSPIYPLSDS ETSACRYPSH SKSQVLLKDR HSRNPSLLGQ DPSPETSPPI CTLKATSFSY LDRTPSLRKR EDQKETVQGA VQDVEGVAAC LPLAQSTPFL GAGSRSVLLS CTGTRAHSLG IREKISAWEG RREASPRMSL CGEKREGPGS EWSVSEGCPS VGCPSVVPSP CSSEKTFDFK GLRRMSRTFS ECSYPETEEE AEALPGRDSL YRLEKRPGRT EPSALLRGHG IRKESSAVLS RIQKIEQALK EQPGRGLPQL PSSCYSVDQG RRKTGTLGTL EEPTGTASVS PSSRAGGVAG VAGEAGPPLD REGSASMKSE TPGNSSSPQL LPPKSSPDPA VNPVPKPKRT FEYEADKNPK TKPSNGLPPS PTPAAPPPLP STPAPPVTRR PKKDMRGHRK SQNRKSFEFE DASSLQSLYP SSPTENGTES QPKFGSKSTL EENAYEDIVG GLPKENPYED VDLKNRRAGR KSQQLSENSL DSLHRMWSPQ DRKYNHPPMQ LSLKSNSQSL RSGNWSERKS HRLPRLPKRH SHDDMMLLAQ LSLPSSPSSL NEDSLSTTSE LLSSRRSRRI PKLVQRINSI YNAKRGKKRL KKLSMSSLET ASLRDENSES ESDSDDRFKA HTQRLVHIQS MLKRAPSYRT LELELLEWQE RELFEYFVVV SLKKKPSRNT YLPEVSYQFP KLDRPTKQMR EAEERLKAIP QFCFPDAKDW LPVSEYSSET FSFMLTGEDG SRRFGYCRRL LPSGKGPRLP EVYCVISRLG CFGLFSKVLD EVERRRGISA ALVYPFMRSL MESPFPAPGK TIKVKTFLPG AGNEVLELRR PMDSRLEHVD FECLFTCLSV RQLIRIFASL LLERRVIFVA DKLSTLSSCS HAVVALLYPF SWQHTFIPVL PASMIDIVCC PTPFLVGLLS SSLPKLKELP VEEALMVNLG SDRFIRQMDD EDTLLPRKLQ AALEQALERK SELISQDSDS DSDDECNTLN GLVSEVFIRF FVETVGHYSL FLTHSEKGER AFQREAFRKS VASKSIRRFL EVFMESQMFA GFIQDRELRK CRAKGLFEQR VEQYLEELPD TEQSGMNKFL RGLGNKMKFL HKKN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ST5 (Suppression of Tumorigenicity 5 (ST5))

Alternative Name: Dennd2b (ST5 Products)

Synonyms: zgc:91902 Protein, wu:fc11b09 Protein, si:ch211-208c6.1 Protein, ST5 Protein, DKFZp459D1230 Protein, hts1 Protein, p126 Protein, dennd2b Protein, DENND2B Protein, HTS1 Protein, 2010004M01Rik Protein, 2610305K15Rik Protein, suppression of tumorigenicity 5 Protein, st5 Protein, ST5 Protein, St5 Protein

Background: DENN domain-containing protein 2B (HeLa tumor suppression 1) (Suppression of tumorigenicity 5 protein),FUNCTION: [Isoform 1]: May be involved in cytoskeletal organization and tumorogenicity. Seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Plays a role in EGFR trafficking from recycling endosomes back to the cell membrane. {ECO:0000250|UniProtKB:P78524}., FUNCTION: [Isoform 2]: Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. {ECO:0000250|UniProtKB:P78524}., FUNCTION: [Isoform 3]: May block ERK2 activation stimulated by ABL1. May alter cell morphology and cell growth. {ECO:0000250|UniProtKB:P78524}.

Molecular Weight: 126.9 kDa

UniProt: Q924W7