PARD3 Protein (AA 1-1333) (Strep Tag)

Catalog No. ABIN3136991

Product Details

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Protein Type: Recombinant

Protein Characteristics: AA 1-1333

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PARD3 protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MKVTVCFGRT RVVVPCGDGR MKVFSLIQQA VTRYRKAVAK DPNYWIQVHR LEHGDGGILD LDDILCDVAD DKDRLVAVFD EQDPHHGGDG TSASFTGTQS PEIFGSELGT NNVSAFQPYQ ATSEIEVTPS VLRANMPLHV RRSSDPALTG LSTSVSDNNF SSEEPSRKNP TRWSTTAGFL KQNTAGSPKT CDRKKDENYR SLPRDPSSWS NQFQRDNARS SLSASHPMVD RWLEKQEQDE EGTEEDSSRV EPVGHADTGL ENMPNFSLDD MVKLVQVPND GGPLGIHVVP FSARGGRTLG LLVKRLEKGG KAEQENLFHE NDCIVRINDG DLRNRRFEQA QHMFRQAMRA RVIWFHVVPA ANKEQYEQLS QREKNNYSPG RFSPDSHCVA NRSVANNAPQ ALPRAPRLSQ PPEQLDAHPR LPHSAHASTK PPAAPALAPP SVLSTNVGSV YNTKKVGKRL NIQLKKGTEG LGFSITSRDV TIGGSAPIYV KNILPRGAAI QDGRLKAGDR LIEVNGVDLA GKSQEEVVSL LRSTKMEGTV SLLVFRQEEA FHPREMNAEP SQMQTPKETK AEDEDVVLTP DGTREFLTFE VPLNDSGSAG LGVSVKGNRS KENHADLGIF VKSIINGGAA SKDGRLRVND QLIAVNGESL LGKANQEAME TLRRSMSTEG NKRGMIQLIV ARRISRCNEL RSPGSPAAPE LPIETELDDR ERRISHSLYS GIEGLDESPT RNAALSRIMG KCQLSPTVNM PHDDTVMIED DRLPVLPPHL SDQSSSSSHD DVGFIMTEAG TWAKATISDS ADCSLSPDVD PVLAFQREGF GRQSMSEKRT KQFSDASQLD FVKTRKSKSM DLVADETKLN TVDDQRAGSP SRDVGPSLGL KKSSSLESLQ TAVAEVTLNG NIPFHRPRPR IIRGRGCNES FRAAIDKSYD KPMVDDDDEG METLEEDTEE SSRSGRESVS TSSDQPSYSL ERQMNGDPEK RDKTERKKDK AGKDKKKDRE KEKDKLKAKK GMLKGLGDMF RFGKHRKDDK MEKMGRIKIQ DSFTSEEDRV RMKEEQERIQ AKTREFRERQ ARERDYAEIQ DFHRTFGCDD ELLYGGMSSY EGCLALNARP QSPREGHLMD TLYAQVKKPR SSKPGDSNRS TPSNHDRIQR LRQEFQQAKQ DEDVEDRRRT YSFEQSWSSS RPASQSGRHS VSVEVQVQRQ RQEERESFQQ AQRQYSSLPR QSRKNASSIS QDSWEQNYAP GEGFQSAKEN PRYSSYQGSR NGYLGGHGFN ARVMLETQEL LRQEQRRKEQ QLKKQPPADG VRGPFRQDVP PSPSQVARLN RLQTPEKGRP FYS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Alternative Name: Pard3 (PARD3 Products)

Synonyms: ASIP Protein, Baz Protein, PAR3 Protein, PAR3alpha Protein, PARD-3 Protein, PARD3A Protein, SE2-5L16 Protein, SE2-5LT1 Protein, SE2-5T2 Protein, Par3 Protein, AA960621 Protein, AI256638 Protein, D8Ertd580e Protein, PAR-3 Protein, Pard3a Protein, asip Protein, bazooka Protein, par-3 Protein, par3 Protein, pard3 Protein, par-3 family cell polarity regulator Protein, partitioning defective 3 homolog Protein, par-3 family cell polarity regulator alpha, b Protein, par-3 family cell polarity regulator S homeolog Protein, PARD3 Protein, Pard3 Protein, LOC100551156 Protein, LOC100473914 Protein, pard3 Protein, pard3ab Protein, pard3.S Protein

Background: Partitioning defective 3 homolog (PAR-3) (PARD-3) (Atypical PKC isotype-specific-interacting protein) (ASIP) (Ephrin-interacting protein) (PHIP),FUNCTION: Adapter protein involved in asymmetrical cell division and cell polarization processes (By similarity). Seems to play a central role in the formation of epithelial tight junctions (By similarity). Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly (PubMed:11839275). The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (By similarity). Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons (By similarity). Involved in Schwann cell peripheral myelination (PubMed:21949390). {ECO:0000250|UniProtKB:Q8TEW0, ECO:0000250|UniProtKB:Q9Z340, ECO:0000269|PubMed:11839275, ECO:0000269|PubMed:21949390}.

Molecular Weight: 149.1 kDa

UniProt: Q99NH2

Pathways: Cell-Cell Junction Organization