TRIM33 Protein (AA 1-1142) (Strep Tag)

Catalog No. ABIN3137005

Product Details

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Protein Type: Recombinant

Protein Characteristics: AA 1-1142

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TRIM33 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MAENKGGGEA ESGGGGSGSA PVTAGAAGPT AQEAEPPLAA VLVEEEEEEG GRAGAEGGAA GPDDGGVAAA SSSSAPAASV PAASVGSAVP GGAASTPAPA AAPAPAPAPA PAPAPAPAPA PAPGSSSGPP LGPPASLLDT CAVCQQSLQS RREAEPKLLP CLHSFCLRCL PEPERQLSVP IPGGSNGDVQ QVGVIRCPVC RQECRQIDLV DNYFVKDTSE APSSSDEKSE QVCTSCEDNA SAVGFCVECG EWLCKTCIEA HQRVKFTKDH LIRKKEDVSE SVGTSGQRPV FCPVHKQEQL KLFCETCDRL TCRDCQLLEH KEHRYQFLEE AFQNQKGAIE NLLAKLLEKK NYVHFAATQV QNRIKEVNET NKRVEQEIKV AIFTLINEIN KKGKSLLQQL ENVTKERQMK LLQQQNDITG LSRQVKHVMN FTNWAIASGS STALLYSKRL ITFQLRHILK ARCDPVPAAN GAIRFHCDPT FWAKNVVNLG NLVIESKPAP GYTPNVVVGQ VPPGTNHISK TPGQINLAQL RLQHMQQQVY AQKHQQLQQM RLQQPPAPIP TTTATTQQHP RQAAPQMLQQ QPPRLISVQT MQRGNMNCGA FQAHQMRLAQ NAARIPGIPR HSAPQYSMMQ PHLQRQHSNP GHAGPFPVVS AHNPINPTSP TTATMANANR GPTSPSVTAI ELIPSVTNPE NLPSLPDIPP IQLEDAGSSS LDNLLSRYIS GSHLPPQPTS TMNPSPGPSA LSPGSSGLSN SHTPVRPPST SSTGSRGSCG SSGRTAEKSA HSFKSDQVKV KQEPGTEEEI CSFSGAVKQE KTEDGRRSAC MLSSPESSLT PPLSTNLHLE SELDTLTGLE NHVKTEPTDI SESCKQSGLS NLVNGKSPIR NLMHRSARIG GDGNSKDDDP NEDWCAVCQN GGDLLCCEKC PKVFHLTCHV PTLLSFPSGD WICTFCRDIG KPEVEYDCDN MQHSKKGKTA QGLSPVDQRK CERLLLYLYC HELSIEFQEP VPVSIPNYYK IIKKPMDLST VKKKLQKKHS QHYQIPDDFV ADVRLIFKNC ERFNEMMKVV QVYADTQEIN LKGDSEVAKA GKAVALYFED KLSEIYSDRT FTPLPEFEQD EDDGEVTEDS DEDFIQPRRK RLKSDERPVH IK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Alternative Name: Trim33 (TRIM33 Products)

Synonyms: TRIM33 Protein, ptc7 Protein, rfg7 Protein, tf1g Protein, tif1g Protein, tifgamma Protein, MGC146665 Protein, tif1gamma Protein, ECTO Protein, PTC7 Protein, RFG7 Protein, TF1G Protein, TIF1G Protein, TIF1GAMMA Protein, TIFGAMMA Protein, ecto Protein, 8030451N04Rik Protein, AI413936 Protein, Ecto Protein, Tif1g Protein, ectodermin Protein, mKIAA1113 Protein, cb1085 Protein, id:ibd2175 Protein, mon Protein, wu:fc17f10 Protein, zgc:136680 Protein, tripartite motif containing 33 Protein, tripartite motif containing 33 L homeolog Protein, tripartite motif-containing 33 Protein, TRIM33 Protein, trim33 Protein, trim33.L Protein, Trim33 Protein

Background: E3 ubiquitin-protein ligase TRIM33 (EC 2.3.2.27) (Ectodermin homolog) (RING-type E3 ubiquitin transferase TRIM33) (Transcription intermediary factor 1-gamma) (TIF1-gamma) (Tripartite motif-containing protein 33),FUNCTION: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity). {ECO:0000250, ECO:0000269|PubMed:16751102}.

Molecular Weight: 123.8 kDa

UniProt: Q99PP7