IFI44L Protein (AA 1-447) (Strep Tag)

Catalog No. ABIN3137014

Product Details

Target: IFI44L (Interferon-Induced Protein 44-Like (IFI44L))

Protein Type: Recombinant

Protein Characteristics: AA 1-447

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This IFI44L protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MKVTARLTWI EEKILEKLLG NASLTLLYQS SAHKNCVSEM TQKYSLQGST MTVFHLEKDV VVGVFILENF PRLVSEKPCT CAWFSLKRNN SSGISALFLN TKVIVDSEEL IIFSLDGLSL SVTPLRGFTL ALNDTVMNGL ELNLGHGFLP VECEIFRVDG IKKNPSFIKK MVTAEQHRGK LLSALRAYKP YKDLVSEVRI LLVGPVGSGK SSFFNSVKSA FQGHLTRQAI VGSDESSITK QYRVYSIKDG KSGETLPFML CDSMGLEEGE EAGLCIDDIP HILQGCVPDR YQFNPCEPMK PKHSPHAASP PLKDRIHCVA FVLHINSVNT LSDKMVAKLK KIRKDVVDCG IGYVALLTNV EEYDEVLDDS FANMTETVTS LSQVQNVQKW LNIPIANILM VSNYASERRL EPMKDILVFA ALRQMLRAAD DALEDLPLED TGNLAPF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: IFI44L (Interferon-Induced Protein 44-Like (IFI44L))

Alternative Name: Ifi44l (IFI44L Products)

Synonyms: C1orf29 Protein, H-28 Protein, H28 Protein, H28-1 Protein, NS1178 Protein, IFI44L Protein, interferon induced protein 44 like Protein, interferon-induced protein 44 like Protein, interferon-induced protein 44-like Protein, IFI44L Protein, Ifi44l Protein, LOC100465846 Protein, LOC100538089 Protein, ifi44l Protein

Background: Interferon-induced protein 44-like [Cleaved into: Minor histocompatibility antigen HA-28 (HLA-HA28) (IFL8)],FUNCTION: Type I interferon-stimulated gene (ISG) that plays a critical role in antiviral and antibacterial activity. During bacterial infection, promotes macrophage differentiation and facilitates inflammatory cytokine secretion (By similarity). Plays a role in the control of respiratory syncycial virus/RSV infection, reducing the ability of the virus to replicate (PubMed:32611756). Acts as a feedback regulator of IFN responses by negatively regulating IKBKB kinase activity through interaction with FKBP5 (By similarity). {ECO:0000250|UniProtKB:Q53G44, ECO:0000269|PubMed:32611756}., FUNCTION: Precursor of the histocompatibility antigen HA-28 in BALB.B mice. More generally, minor histocompatibility antigens refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC molecules and its expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD). More specifically, HA-28 minor antigen is transcribed in the BALB.B donor but not in host C57BL/6 cells. HA-28 is presented to the donor BALB.B cell surface by Kb MHC. This complex HA-28/Kb MHC elicits cytotoxic T-cell response in C57BL/6 mice immunized with BALB.B spleen cells. It induces C57BL/6 mice cells recognition and lysis by CD8 T-cell from BALB.B mice. {ECO:0000269|PubMed:11021531}.

Molecular Weight: 49.7 kDa

UniProt: Q9BDB7