PHF19 Protein (AA 1-578) (Strep Tag)

Catalog No. ABIN3137054

Product Details

Target: PHF19 (PHD Finger Protein 19 (PHF19))

Protein Type: Recombinant

Protein Characteristics: AA 1-578

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PHF19 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: METQALEPGT LEAFGATSPN KGGLSKTKKN FKDLMSKVTE GQFVLCRWTD GLYYLGKIKR VSSPKQSCLV TFEDNSKYWV LWKDIQHAGV PGEEPKCDVC MGKTSGPMNE ILICGKCGLG YHQQCHIPIA VDANWPLLTH WFCRRCIFAL AVRKGGALKK GAIAKTLQAV KMVLSYQPEE LDWDSPHRTN QQQCYCYCGG PGEWYLRMLQ CYRCRQWFHE ACTQCLSEPM VFGDRFYLFF CSVCNQGPEY IERLPLRWVD IVHLALYNLG VQSKKRYFDF EEILAFVNHH WELLQLGKLT STPMTERGPH LLNALNSYKS RFLCGKEIKK KKCIFRLRIR VPPAPPGKLL PDRALMPSDK GTSELLRKKG KSKPGLLPQE PQQQKRRVYR RKRSKFLLED AIPSSDFTSA WSTDHHLASI FDFTLDEIQS LKSGSSGQTF FSDVDSTDAA STSGSASTSL SYDSRWTVGS RKRKLTAKVH RPLRAKQRAA ELEGRCASDS NAEGAVGPEQ PDEGIDSHTL ESISGDDSSL SHLKSSITNY FGAAGRLACG EKYRVLARRV TPEGKVQYLL EWEGTTPY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PHF19 (PHD Finger Protein 19 (PHF19))

Alternative Name: Phf19 (PHF19 Products)

Synonyms: si:dkeyp-51b7.1 Protein, pcl3 Protein, MGC147603 Protein, MTF2L1 Protein, PCL3 Protein, TDRD19B Protein, 3110009G19Rik Protein, 3321402G02Rik Protein, PHD finger protein 19 Protein, Phf19 Protein, PHF19 Protein, phf19 Protein

Background: PHD finger protein 19 (Polycomb-like protein 3),FUNCTION: Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity (PubMed:23104054, PubMed:23160351, PubMed:22438827, PubMed:23273982). Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing (PubMed:23160351). Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal (PubMed:22438827). Also binds histone H3 dimethylated at 'Lys-36' (H3K36me2) (By similarity). {ECO:0000250|UniProtKB:Q5T6S3, ECO:0000269|PubMed:22438827, ECO:0000269|PubMed:23104054, ECO:0000269|PubMed:23160351, ECO:0000269|PubMed:23273982}.

Molecular Weight: 65.2 kDa

UniProt: Q9CXG9

Pathways: Stem Cell Maintenance