N-Glycanase 1 Protein (NGLY1) (AA 2-651) (His tag)

Catalog No. ABIN3137268

Product Details

Target: N-Glycanase 1 (NGLY1)

Protein Type: Recombinant

Protein Characteristics: AA 2-651

Origin: Mouse

Source: Insect Cells

Purification tag / Conjugate: This N-Glycanase 1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ASATLGSSSS SASPAVAELC QNTPETFLEA SKLLLTYADN ILRNPSDEKY RSIRIGNTAF STRLLPVRGA VECLFEMGFE EGETHLIFPK KASVEQLQKI RDLIAIERSS RLDGSSKKVQ FSQHPAAAKL PLEQSEDPAG LIRHSGNQTG QLPSLPSAPM VVGDSTILKV LQSNIQHVQL YENPVLQEKA LTCIPVSELK RKAQEKLFRA RKLDKGTNVS DEDFLLLELL HWFKEEFFRW VNNIVCSKCG GETRSRDEAL LPNDDELKWG AKNVENHYCD ACQLSNRFPR YNNPEKLLET RCGRCGEWAN CFTLCCRALG FEARYVWDYT DHVWTEVYSP SQQRWLHCDA CEDVCDKPLL YEIGWGKKLS YIIAFSKDEV VDVTWRYSCK HDEVMSRRTK VKEELLRETI NGLNKQRQLS LSESRRKELL QRIIVELVEF ISPKTPRPGE LGGRVSGSLA WRVARGETGL ERKEILFIPS ENEKISKQFH LRYDIVRDRY IRVSDNNINI SGWENGVWKM ESIFRKVEKD WNMVYLARKE GSSFAYISWK FECGSAGLKV DTVSIRTSSQ SFESGSVRWK LRSETAQVNL LGDKNLRSYN DFSGATEVTL EAELSRGDGD VAWQHTQLFR QSLNDSGENG LEIIITFNDL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ngly1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: N-Glycanase 1 (NGLY1)

Alternative Name: Ngly1 (NGLY1 Products)

Synonyms: CDG1V Protein, PNG1 Protein, PNGase Protein, 1110002C09Rik Protein, Png1 Protein, im:6895151 Protein, zgc:110561 Protein, AtPNG1 Protein, K6M13.12 Protein, K6M13_12 Protein, peptide-N-glycanase 1 Protein, N-glycanase 1 Protein, N-glycanase 1 S homeolog Protein, peptide-N-glycanase 1 Protein, Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Protein, similar to S. cerevisiae PNG1 (YPL096W) peptide:N-glycanase de-N-glycosylation enzyme Protein, NGLY1 Protein, ngly1.S Protein, ngly1 Protein, Ngly1 Protein, PNG1 Protein, png-1 Protein

Background: Specifically deglycosylates the denatured form of N-linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl-glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high-mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins. {ECO:0000269|PubMed:11562482, ECO:0000269|PubMed:12606569, ECO:0000269|PubMed:15358861}.

Molecular Weight: 75.1 kDa Including tag.

UniProt: Q9JI78

Pathways: Cell RedoxHomeostasis, SARS-CoV-2 Protein Interactome