ERCC6L2 Protein (AA 1-1537) (Strep Tag)

Catalog No. ABIN3137278

Product Details

Target: ERCC6L2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like 2 (ERCC6L2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1537

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ERCC6L2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MDISAPQSRA DSRKDRWCPG ERCLAPSLDN KKLCEASIKS ITVDGNGKPF AVVLYPDFQE KTIPLQRLQE VKSTKDYSRS LIFDDKDLEK PYFPDRKIPS LASAFQLSED GDSIPYTINR YLRDYQREGA QFLYRHYIEG RGCILGDDMG LGKTIQVISF LAAVLHKKGT REDIENNMPE FLLKSMKKKP PSTAKKMFLI VAPLSVLYNW KDELDTWGYF RVTVLHGSKK DNELLRLKQR KCEIALTTYE TLRLCLEELN SLEWSAIIVD EAHRIKNPKA RVTEVMKAVK CKVRIGLTGT VLQNNMKELW CVMDWAVPGL LGSRIHFKKQ FSDPVEHGQR HTATKRELAT GRKAMHRLAK KMSGWFLRRT KTLIKGQLPK KEDRMVYCSL TDFQKAVYQT VLETEDVALI LTSSQPCTCG SGQKRRKCCY KTNSRGDTVR TLCLSYLTVL QKVANHVALL QAASTSKHQE TVIKRICDRV FSRFPDFVQK SKDAAFETLS DPKYSGKMKV LQQLLNHFRK QRDKVLLFSF STKLLDVLQQ YCMASGLDYR RLDGSTKSEE RLKIVKEFNS SQDVNICLVS TMAGGLGLNF VGANVVILFD PTWNPANDLQ AVDRAYRIGQ CRDVKVLRLI SLGTVEEIMY LRQVYKQQLH CVVVGSENAK RYFEAVQGSK EHRGELFGVH NLFKLRSQGS CLTRDILERE GQVEAGIMTA TTWLKGEPSA QELETPRDPD CQEPTDVCEL YSDISDEESV GHSLGKTDKH KFSDTSRTPG FPAQLTLLQC GFSKLFEAKY KSDQDGDGNP VPSDGSSDEQ PMCLSAEARQ AARQKTWDSV CTSEHQKSDN IQTPDEKCVS DKSEKTLEQN VSSESDDETK DHRTAGHHCM GQGDTESEDS DVIFPTQYPT QRIPKNHIRF KLLLGESEDS EAENPVKVNH GDDRQNSGRG NGPVPNLLCL ENMTSKSVRK RKGTDDISDE SDDIDMFPKS RIRKQRATTS LKFKRKKENK RKLDNSPVTA KEANQVCAAD GDRSSQVIED FSSSDDNLSL SHLSFTKLSH RAETVKDKIS LSPKLPGPDK KNNTFISRKP PSFLNEGVIS QEQICNSMDK ILDGVQEVAY IHSNQNVIGS SRAENHMSRW ATRDVFELKQ FSQLPANVAV CSSKTYKTQV KANIVSPTEK DQPPSDGGIS SPLYVSHPVV QKKKDVYRTN HTTFIIGETP RGIRRKQFEE MASYYKLPVK EFAEQVTRAT SEERQKMLRD FYSLQHPEVK EFFVNSASEL IKSVHKKEER VRNKSKEKES LLKENPSNDS TLSCYDSTNK MSQVYNRKIC EGKSVRSQNH VFHREDTFSS DAEINKSPVS FTEELHSERK DHTPKDTTTV FCPNSNSEAL EAELGNSPGR QWDLTGACGS RNRPLFKLRN KRVENPGSEN TPEDGLLGDT SILNDLFKSH GEGPTQLPKN VLSGPVAKAK QKPKDFWDIL NEQNDDSLSK LTDLAVIETL CTKAPSTSAS KRKDELEASL WKANEKFLWK TLSSDVDDES ISNTERE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ERCC6L2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6-Like 2 (ERCC6L2))

Alternative Name: Ercc6l2 (ERCC6L2 Products)

Synonyms: 0610007P08Rik Protein, 1700019D06Rik Protein, 9330134C04Rik Protein, Rad26l Protein, Sr278 Protein, C9orf102 Protein, RAD26L Protein, SR278 Protein, C8H9orf102 Protein, RGD1561537 Protein, si:ch211-248d20.2 Protein, BMFS2 Protein, CZH9ORF102 Protein, excision repair cross-complementing rodent repair deficiency, complementation group 6 like 2 Protein, ERCC excision repair 6 like 2 Protein, excision repair cross-complementation group 6-like 2 Protein, excision repair cross-complementation group 6 like 2 Protein, Ercc6l2 Protein, ERCC6L2 Protein, ercc6l2 Protein

Background: DNA excision repair protein ERCC-6-like 2 (EC 3.6.4.-) (DNA repair and recombination protein RAD26-like),FUNCTION: May be involved in early DNA damage response. {ECO:0000250}.

Molecular Weight: 173.9 kDa

UniProt: Q9JIM3