SH3GLB1 Protein (AA 1-365) (His tag)
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- Target See all SH3GLB1 Proteins
- SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-365
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Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This SH3GLB1 protein is labelled with His tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Sequence
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MNIMDFNVKK LAADAGTFLS RAVQFTEEKL GQAEKTELDA HLENLLSKAE CTKIWTEKIM KQTEVLLQPN PNARIEEFVY EKLDRKAPSR INNPELLGQY MIDAGTEFGP GTAYGNALIK CGETQKRIGT ADRELIQTSA LNFLTPLRNF IEGDYKTIAK ERKLLQNKRL DLDAAKTRLK KAKAAETKSS SEQELRITQS EFDRQAEITR LLLEGISSTH AHHLRCLNDF VEAQMTYYAQ CYQYMLDLQK QLGSFPSNYL SNNNQTSGTP VPYALSNAIG PSAQASTGSL VITCPSNLND LKESSNNRKA RVLYDYDAAN STELSLLADE VITVFSVVGM DSDWLMGERG NQKGKVPITY LELLN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Sh3glb1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product SH3GLB1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- SH3GLB1 (SH3-Domain GRB2-Like Endophilin B1 (SH3GLB1))
- Alternative Name
- Sh3glb1 (SH3GLB1 Products)
- Synonyms
- im:5777505 Protein, wu:fa08f03 Protein, wu:fb13e12 Protein, MGC79086 Protein, sh3glb1 Protein, wu:fc55h11 Protein, wu:fj98d09 Protein, AA409932 Protein, AI314629 Protein, AU015566 Protein, Bif-1 Protein, mKIAA0491 Protein, PPP1R70 Protein, dJ612B15.2 Protein, SH3-domain GRB2-like endophilin B1b Protein, SH3 domain GRB2 like, endophilin B1 S homeolog Protein, SH3 domain GRB2 like, endophilin B1 Protein, SH3 domain containing GRB2 like, endophilin B1 Protein, SH3-domain GRB2-like endophilin B1a Protein, SH3-domain GRB2-like B1 (endophilin) Protein, SH3 domain -containing GRB2-like endophilin B1 Protein, SH3 domain containing GRB2 like endophilin B1 Protein, sh3glb1b Protein, sh3glb1.S Protein, sh3glb1 Protein, SH3GLB1 Protein, sh3glb1a Protein, Sh3glb1 Protein
- Background
- May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells (PubMed:17086176). May recruit other proteins to membranes with high curvature. May promote membrane fusion (By similarity). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (PubMed:24523556). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Isoform 2 acts antiapoptotic in neuronal cells, involved in maintenance of mitochondrial morphology and promotes neuronal viability (PubMed:24523556). {ECO:0000250|UniProtKB:Q9Y371, ECO:0000269|PubMed:17086176, ECO:0000269|PubMed:24523556}.
- Molecular Weight
- 41.8 kDa Including tag.
- UniProt
- Q9JK48
- Pathways
- Autophagy
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