TP53INP1 Protein (AA 1-239) (Strep Tag)

Catalog No. ABIN3137372

Product Details

Target: TP53INP1 (Tumor Protein P53 Inducible Nuclear Protein 1 (TP53INP1))

Protein Type: Recombinant

Protein Characteristics: AA 1-239

Origin: Mouse

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This TP53INP1 protein is labelled with Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequence: MFQRLNKMFV GEVTTSSSQE PEFSEKEDDE WILVDFIDTC PGFSAEEEEE DEDIGEESSA EHTSVFSCLP ASLECLTDTS DSCFLQFESC PMEESWFITP PPCFTAGGLT TIKVETSPME NLLIEHPSMS VYAVHNSCPG LSEASCGNDE YNSSGPRMEA QSEMGKHIHC CVAALAAQAT FLEQPKSFRP SQWIKGHSER QSLNRNGLRR QNLTRDCHTR QMKHSGWVVH QPCPRQYNY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: TP53INP1 (Tumor Protein P53 Inducible Nuclear Protein 1 (TP53INP1))

Alternative Name: Trp53inp1 (TP53INP1 Products)

Synonyms: cb1036 Protein, wu:fb70a09 Protein, wu:fi13h08 Protein, zgc:158431 Protein, TP53INP1 Protein, 2700057G22Rik Protein, SIP Protein, SIP18 Protein, SIP27 Protein, Stinp Protein, Teap Protein, TP53DINP1 Protein, TP53INP1A Protein, TP53INP1B Protein, p53DINP1 Protein, Trp53inp1 Protein, tumor protein p53 inducible nuclear protein 1 Protein, tumor protein p53 inducible nuclear protein 1 S homeolog Protein, transformation related protein 53 inducible nuclear protein 1 Protein, TP53INP1 Protein, tp53inp1.S Protein, tp53inp1 Protein, Trp53inp1 Protein, Tp53inp1 Protein

Background: Tumor protein p53-inducible nuclear protein 1 (Stress-induced protein) (Thymus-expressed acidic protein) (TEAP) (p53-dependent damage-inducible nuclear protein 1) (p53DINP1),FUNCTION: Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. {ECO:0000269|PubMed:11557757, ECO:0000269|PubMed:16044147, ECO:0000269|PubMed:19118006, ECO:0000269|PubMed:21339733}.

Molecular Weight: 26.9 kDa

UniProt: Q9QXE4