MAD1L1 Protein (AA 1-717) (His tag)
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- Target See all MAD1L1 Proteins
- MAD1L1 (MAD1 Mitotic Arrest Deficient-Like 1 (MAD1L1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-717
- Origin
- Mouse
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Source
- Insect Cells
- Purification tag / Conjugate
- This MAD1L1 protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MEDLGENTTV LSSLRSLNNF ISQRMEGTSG LDVSTSASGS LQKQYEYHMQ LEERAEQIRS KSYLIQVERE KMQMELSHKR ARVELERAAS TNARNYEREV DRNQELLARI RQLQECEATA EEKMREQLER HRLCKQNLDA VSQQLREQED SLASAREMIS SLKGRVSELQ LSAMDQKVQV KRLESEKQEL KEQLELQQRK WQEANQKIQE LQASQDERAE HEQKIKDLEQ KLCLQEQDAA VVKSMKSELM RMPRMERELK RLHEENTHLR EMKETNGLLT EELEGLQRKL SRQEKMQEAL VDLELEKEKL LAKLQSWENL DQTMGLNLRT PEDLSRFVVE LQQRELTLKE KNNSITSSAR GLEKVQQQLQ DEVRQANAQL LEERKKRETH EALARRLQKR NALLTKERDG MRAILGSYDS ELTQTEYSTQ LTQRLWEAED MVQKVHAHSS EMEAQLSQAL EELGVQKQRA DTLEMELKML KAQTSSAESS FSFCKEEVDA LRLKVEELEG ERSRLEQEKQ VLEMQMEKLT LQGDYNQSRT KVLHMSLNPI SMARQRQHED HDRLQEECER LRGLVHALER GGPIPADLEA ASSLPSSKEV AELRKQVESA ELKNQRLKEV FQTKIQEFRK VCYTLTGYQI DVTTESQYRL TSRYAEHQTD CLIFKATGPS GSKMQLLETE FSRSVPELIE LHLLQQDSIP AFLSALTIEL FSRQTSI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Mad1l1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product MAD1L1 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- MAD1L1 (MAD1 Mitotic Arrest Deficient-Like 1 (MAD1L1))
- Alternative Name
- Mad1l1 (MAD1L1 Products)
- Synonyms
- MAD1 Protein, PIG9 Protein, TP53I9 Protein, TXBP181 Protein, AI173502 Protein, AW550425 Protein, Mad1 Protein, zgc:56571 Protein, MAD1-like protein 1 Protein, mitotic arrest deficient 1 like 1 Protein, MAD1 mitotic arrest deficient like 1 Protein, MAD1 mitotic arrest deficient 1-like 1 Protein, deafness, autosomal recessive 19 Protein, MAD1L1 Protein, Mad1l1 Protein, mad1l1 Protein, DFNB19 Protein
- Background
- Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding (By similarity). {ECO:0000250}.
- Molecular Weight
- 84.5 kDa Including tag.
- UniProt
- Q9WTX8
- Pathways
- M Phase
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