Neuregulin 1 (NRG1) (AA 1-525) (Active) protein (His tag)

Details for Product No. ABIN3216389, Supplier: Log in to see
Protein Name
  • 6030402G23Rik
  • ARIA
  • D230005F13Rik
  • GGF
  • GGFII
  • HRG
  • HRGalpha
  • Hgl
  • NDF
  • SMDF
  • GGF2
  • HGL
  • HRG1
  • HRGA
  • MST131
  • NRG-1
  • heregulin
  • nrg1-A
  • pro-neuregulin-1
  • NRG1
  • wu:fm70e01
  • DKFZp459A1838
  • neuregulin 1
  • neuregulin 1 L homeolog
  • Nrg1
  • NRG1
  • nrg1.L
  • nrg1
Protein Characteristics
AA 1-525
5
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Origin
Human
37
8
5
3
1
Source
Human Cells
28
14
6
2
2
1
Protein Type
Recombinant
Biological Activity
Active
Purification tag / Conjugate
This Neuregulin 1 protein is labelled with His tag.
Application
Functional Studies (Func), SDS-PAGE (SDS)
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Characteristics The secreted recombinant human HPRG comprises 518 amino acids with a predicted molecular mass of 59 kDa. As a result of glycosylation, rhHPRG migrates as an approximately 75-80 kDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human HPRG (NP_000403.1) (Met 1-Lys 525) with a C-terminal polyhistidine tag was expressed.
Predicted N-Term: Val 19
Purity > 97 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
Biological Activity Comment Measured by its ability to support the adhesion of MOLT-4 human acute lymphoblastic leukemia cells. (Lamb-Wharton, R.J. and W.T. Morgen, 1993, Cell Immunol. 152: 544) . Human HPRG immobilized at 1 μg/ml (100 μl/well) will induce >65% MOLT4 cell adhesion (1 x 10E5 cells/well) in the presence of 7.5 μg/ml Concanvalin A.
Background Synonyms: HPRG,HRGP,THPH11
Molecular Weight 59 kDa, 75-80 kDa
NCBI Accession NP_000403
Research Area Cardiovascular, Angiogenesis
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Expiry Date 12 months
Supplier Images
 image for Neuregulin 1 (NRG1) (AA 1-525) (Active) protein (His tag) (ABIN3216389) Neuregulin 1 (NRG1) (AA 1-525) (Active) protein (His tag)
Background publications Sørensen, Krogh-Pedersen, Petersen: "Determination of the disulphide bridge arrangement of bovine histidine-rich glycoprotein." in: FEBS letters, Vol. 328, Issue 3, pp. 285-90, 1993

Sørensen, Krogh-Pedersen, Petersen: "Determination of the disulphide bridge arrangement of bovine histidine-rich glycoprotein." in: FEBS letters, Vol. 328, Issue 3, pp. 285-90, 1993 (PubMed).

Koide, Foster, Yoshitake, Davie: "Amino acid sequence of human histidine-rich glycoprotein derived from the nucleotide sequence of its cDNA." in: Biochemistry, Vol. 25, Issue 8, pp. 2220-5, 1986 (PubMed).