Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-393) Protein

Details for Product No. ABIN3216428, Supplier: Log in to see
Protein Name
  • Mek1
  • CFC3
  • MAPKK1
  • MEK1
  • MKK1
  • PRKMK1
  • MEKK1
  • Prkmk1
  • mek-2
  • si:ch211-242m18.2
  • wu:fj56a12
  • wu:fj61b01
  • zgc:56557
  • ATMEK1
  • F20B18.180
  • F20B18_180
  • MAP kinase/ ERK kinase 1
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE
  • MITOGEN ACTIVATED PROTEIN KINASE KINASE 1
  • NMAPKK
  • MEK
  • mitogen activated protein kinase kinase 1
  • mitogen-activated protein kinase kinase 1
  • Map2k1
  • MAP2K1
  • map2k1
  • MEK1
Protein Characteristics
AA 1-393
6
4
1
1
1
1
1
1
1
Origin
Mouse (Murine)
11
4
1
1
1
1
1
1
Source
Baculovirus infected Insect Cells
6
4
4
3
2
1
1
Protein Type
Recombinant
Application
SDS-PAGE (SDS)
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Characteristics The recombinant mouse MAP2K1 consists of 395 amino acids and predicts a molecular mass of 43.6 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the mouse MAP2K1 (NP_032953.1) (Met1-Ile393) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Predicted N-Term: Gly
Purity > 90 % as determined by SDS-PAGE
Endotoxin Level < 1.0 EU per μg of the protein as determined by the LAL method
ProductDetails: Biological Activity Comment Kinase activity untested
Background Synonyms: MAPKK1,Mek1,MEKK1,Prkmk1
NCBI Accession NP_032953
Research Area Kinases/Phosphatases, Phospho-specific antibodies
Pathways MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Buffer Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handling Advice Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Storage -20 °C,-80 °C
Storage Comment Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Expiry Date 12 months
Supplier Images
 image for Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-393) Protein (ABIN3216428) Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-393) protein
Background publications Wissing, Jänsch, Nimtz, Dieterich, Hornberger, Kéri, Wehland, Daub: "Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry." in: Molecular & cellular proteomics : MCP, Vol. 6, Issue 3, pp. 537-47, 2007 (PubMed).

Mukherjee, Keitany, Li, Wang, Ball, Goldsmith, Orth: "Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation." in: Science (New York, N.Y.), Vol. 312, Issue 5777, pp. 1211-4, 2006

Mukherjee, Keitany, Li, Wang, Ball, Goldsmith, Orth: "Yersinia YopJ acetylates and inhibits kinase activation by blocking phosphorylation." in: Science (New York, N.Y.), Vol. 312, Issue 5777, pp. 1211-4, 2006 (PubMed).

Duesbery, Webb, Leppla, Gordon, Klimpel, Copeland, Ahn, Oskarsson, Fukasawa, Paull, Vande Woude: "Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor." in: Science (New York, N.Y.), Vol. 280, Issue 5364, pp. 734-7, 1998

Duesbery, Webb, Leppla, Gordon, Klimpel, Copeland, Ahn, Oskarsson, Fukasawa, Paull, Vande Woude: "Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor." in: Science (New York, N.Y.), Vol. 280, Issue 5364, pp. 734-7, 1998 (PubMed).

Seger, Seger, Lozeman, Ahn, Graves, Campbell, Ericsson, Harrylock, Jensen, Krebs: "Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases." in: The Journal of biological chemistry, Vol. 267, Issue 36, pp. 25628-31, 1993

Seger, Seger, Lozeman, Ahn, Graves, Campbell, Ericsson, Harrylock, Jensen, Krebs: "Human T-cell mitogen-activated protein kinase kinases are related to yeast signal transduction kinases." in: The Journal of biological chemistry, Vol. 267, Issue 36, pp. 25628-31, 1993 (PubMed).