phospholipase C beta 2 (PLCb2) (Active) Protein

Details for Product No. ABIN411911, Supplier: Log in to see
Protein Name
  • AI550384
  • B230205M18Rik
  • B230399N12
  • phospholipase C, beta 2
  • phospholipase C beta 2
  • Plcb2
  • PLCB2
Origin
Human
5
2
1
Source
Baculovirus infected Insect Cells
3
3
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >79% Pure active Recombinant PKC beta II.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥79 %
Background Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Greenham determined the genomic structure of the PRKCβ gene, which consists of 18 exons spanning 375 kb (1). PRKCβ has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Leitges found that the 2 isoforms, PRKCβ1 and PRKCβ2, play an important role in B-cell activation and may be functionally linked to Bruton tyrosine kinase in antigen receptor-mediated signal transduction (2). Su proposed that PRKCβ inhibitors and inhibitors of other PRKC isoforms may be effective in treating disorders characterized by dysregulated NFKB survival signaling (3). Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress (4).
Alternate Names: PKC, Protein kinase C beta II
Molecular Weight ~105.0 kDa
Gene ID 5579
NCBI Accession NM_002738
Pathways WNT Signaling, Thyroid Hormone Synthesis, CXCR4-mediated Signaling Events, G-protein mediated Events, Thromboxane A2 Receptor Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 339 nmol phosphate incorporated into CREBtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for phospholipase C beta 2 (PLCb2) (Active) protein (ABIN411911) phospholipase C beta 2 (PLCb2) (Active) protein
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