Checkpoint Kinase 2 (CHEK2) (Active) Protein

Details for Product No. ABIN411919, Supplier: Log in to see
Protein Name
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • CHK-2
  • checkpoint kinase 2
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • OPN2SW
Origin
Human
10
2
Source
Baculovirus infected Insect Cells
4
3
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant CHK2.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background Chk2 is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and Schizosaccharomyces pombe Cds1 protein kinases required for the DNA damage and replication checkpoints. Chk2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage, the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). In vitro, Chk2 phosphorylates p53 on Ser-20 and dissociated preformed complexes of p53 with Mdm2, a protein that targets p53 for degradation (2). In vivo, ectopic expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization. Thus, in response to DNA damage, Chk2 stabilizes the p53 tumor suppressor protein leading to cell cycle arrest in G1. Chk2 is directly phosphorylated by ATM in response to ionizing radiation (3). The phosphorylation occurs in the Ser-Gln/Thr-Gln (SQ/TQ) cluster domain (SCD) on Chk2, which contains seven SQ/TQ motifs, and Thr68 is the major in vitro phosphorylation site by ATM.
Alternate Names: CHK, CHK2 checkpoint homolog
Molecular Weight 88.0 kDa
Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 1138 nmol phosphate incorporated into CHKtide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Checkpoint Kinase 2 (CHEK2) (Active) protein (ABIN411919) Checkpoint Kinase 2 (CHEK2) (Active) protein
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