Calcium/calmodulin-Dependent Protein Kinase ID (CAMK1D) (Active) Protein

Details for Product No. ABIN411920, Supplier: Log in to see
Protein Name
  • CKLiK
  • CaM-K1
  • CaMKID
  • A630059D12Rik
  • CaMKIdelta
  • E030025C11Rik
  • CAMK1D
  • camk1d
  • zgc:158713
  • RGD1560691
  • cam-ki
  • camk1d.L
  • calcium/calmodulin dependent protein kinase ID
  • calcium/calmodulin-dependent protein kinase ID
  • calcium/calmodulin-dependent protein kinase 1Db
  • calcium/calmodulin dependent protein kinase ID S homeolog
  • CAMK1D
  • Camk1d
  • camk1db
  • LOAG_08089
  • camk1d.S
Origin
Human
11
1
Source
Baculovirus infected Insect Cells
4
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Purpose >90% Pure active Recombinant CAMK1d.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background Calcium/calmodulin-dependent protein kinase ID (CAMK1D), or a novel Ca2+/calmodulin-dependent kinase I-like kinase (CKLiK), showed kinase activity and that the activity was enhanced by Ca(2+) and calmodulin. Using a novel antibody generated against the C-terminus of CKLiK, CKLiK was detected in CD34+-derived neutrophils and eosinophils, as well as in mature peripheral blood granulocytes. Activation of human granulocytes by N-formyl-methionyl-leucyl-phenylalanine (fMLP) and platelet-activating factor (PAF), but not the phorbol ester PMA (phorbol 12-myristate-13-acetate), resulted in induction of CKLiK activity, in parallel with a rise of intracellular [Ca2+]. Furthermore, fMLP-induced neutrophil migration on albumin-coated surfaces was perturbed, as well as β2-integrin-mediated adhesion. These findings suggest a critical role for CKLiK in modulating chemoattractant-induced functional responses in human granulocytes (1). Also, CAMK1D exhibits Ca(2+)/CaM-dependent activity that is enhanced (approximately 30-fold) in vitro by phosphorylation of its Thr180 by CaM-K kinase (CaM-KK)α, consistent with detection of CAMK1D-activating activity in HeLa cells. Transiently expressed CAMK1D exhibited enhanced protein kinase activity in HeLa cells without ionomycin stimulation. This sustained activation of CAMK1D was completely abolished by Thr180Ala mutation and inhibited by CaM-KK inhibitor, STO-609, indicating a functional CaM-KK/ CAMK1D cascade in HeLa cells.
Alternate Names: CAMK, Calcium/calmodulin-dependent protein kinase type I delta chain
Molecular Weight 60.0 kDa
Gene ID 8536
UniProt Q14012
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 138 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Calcium/calmodulin-Dependent Protein Kinase ID (CAMK1D) (Active) protein (ABIN411920) Calcium/calmodulin-Dependent Protein Kinase ID (CAMK1D) (Active) protein
Did you look for something else?