Protein Kinase C, zeta (PRKCZ) (Active) Protein

Details for Product No. ABIN411925, Supplier: Log in to see
Protein Name
  • TPKC
  • PKCzeta
  • prkcz-A
  • pkc-zeta
  • PRKCZ
  • pkc2
  • PKC-ZETA
  • PKC2
  • AI098070
  • C80388
  • Pkcz
  • R74924
  • aPKCzeta
  • zetaPKC
  • 14-3-3-zetaisoform
  • r14-3-3
  • pkcz
  • si:ch211-150o23.4
  • protein kinase C zeta L homeolog
  • protein kinase C zeta
  • protein kinase C, zeta
  • prkcz.L
  • PRKCZ
  • prkcz
  • Prkcz
Origin
Human
12
3
1
Source
Baculovirus infected Insect Cells
5
4
2
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant PKC zeta.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background PKCζ (PKC zeta) is an atypical isoform of the PKC family. PKCζ is found in both particulate and soluble fractions and cannot be activated by phorbol ester. Treatment of cells with phorbol ester which activates PKCα, γ, δ, and ε isoforms in NIH3T3 cells significantly reduced proliferation of cells. Overexpression of PKCζ and subsequent phorbol ester treatment abolished phorbol ester-induced reduction in cell proliferation (1). Overexpression of PKCζ also potentiated phorbol ester-induced mitogen-activated protein (MAP) kinase activation in a PKC-dependent manner. The effects of PKCζ overexpression on proliferation and MAP kinase activation are proportional to the levels of PKCζ expression. PKCζ as an upstream modulator of p70S6K, an important regulator of cell proliferation (2). Kinase-inactive PKCζ mutant antagonized activation of p70S6K by epidermal growth factor, PDK-1, and activated Cdc42 and PI3-K. Overexpression of a constitutively active PKCζ mutant (myristoylated PKCζ [myr-PKCzeta]) only modestly activated p70S6K but this mutant cooperated with PDK-1 for the activation of p70S6K. PDK-1-induced activation of a C-terminal truncation mutant of p70S6K was also enhanced by myr-PKCζ. p70S6K can associate with both PDK-1 and PKCz in vivo in a growth factor-independent manner, while PDK-1 and PKCζ can also associate with each other, suggesting the existence of a multimeric PI3-K signalling complex.
Alternate Names: PKC, PKC, Protein kinase C zeta
Molecular Weight 93.0 kDa
Gene ID 5590
UniProt Q05513
Pathways NF-kappaB Signaling, RTK Signaling, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 93 nmol phosphate incorporated into CREBtide per minute per mg protein at 300C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 30 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Protein Kinase C, zeta (PRKCZ) (Active) protein (ABIN411925) Protein Kinase C, zeta (PRKCZ) (Active) protein
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