Hemopoietic Cell Kinase (HCK) (Active) Protein

Details for Product No. ABIN411927, Supplier: Log in to see
Protein Name
  • laloo
  • HCK
  • MGC107870
  • JTK9
  • p59Hck
  • p61Hck
  • AI849071
  • Bmk
  • Hctk
  • p56Hck
  • hemopoietic cell kinase
  • tklal-a
  • HCK
  • hck
  • Hck
Origin
Human
12
2
1
Source
Baculovirus infected Insect Cells
6
2
2
1
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant HCK.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background HCK, a protein-tyrosine kinase, belongs to SRC family members (1). Ziegler et al. found that expression of HCK may be limited to certain hemopoietic cells and is especially prominent in cells of myeloid lineage, particularly mature granulocytes and monocytes (2). Therefore, Quintrell et al. designated the gene HCK (pronounced 'hick') for hemopoietic cell kinase. They described the nucleotide sequence of a cDNA clone and the distribution of RNA transcribed from HCK among various hemopoietic cells. They assigned the HCK gene to 20q11-q12. Since this region is affected by interstitial deletions in some acute myeloid leukemias and myeloproliferative disorders, they suggested that damage to HCK may contribute to the pathogenesis of these conditions (3).
Alternate Names: HCK, Tyrosine-protein kinase, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck
Molecular Weight 57.0 kDa
Gene ID 3055
Pathways Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 171 nmol phosphate incorporated into poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Hemopoietic Cell Kinase (HCK) (Active) Protein (ABIN411927) Hemopoietic Cell Kinase (HCK) (Active) protein
Background publications Piccardoni, Manarini, Federico, Bagoly, Pecce, Martelli, Piccoli, Totani, Cerletti, Evangelista: "SRC-dependent outside-in signalling is a key step in the process of autoregulation of beta2 integrins in polymorphonuclear cells." in: The Biochemical journal, Vol. 380, Issue Pt 1, pp. 57-65, 2004 (PubMed).

Ziegler, Marth, Lewis, Perlmutter: "Novel protein-tyrosine kinase gene (hck) preferentially expressed in cells of hematopoietic origin." in: Molecular and cellular biology, Vol. 7, Issue 6, pp. 2276-85, 1987 (PubMed).

Quintrell, Lebo, Varmus, Bishop, Pettenati, Le Beau, Diaz, Rowley: "Identification of a human gene (HCK) that encodes a protein-tyrosine kinase and is expressed in hemopoietic cells." in: Molecular and cellular biology, Vol. 7, Issue 6, pp. 2267-75, 1987 (PubMed).