Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (Active) Protein

Details for Product No. ABIN411947, Supplier: Log in to see
Protein Name
  • CAMK4
  • camk4-A
  • CaMK IV
  • CaMK-GR
  • IV
  • caMK
  • A430110E23Rik
  • AI666733
  • CaMKIV
  • CaMKIV/Gr
  • D18Bwg0362e
  • Ccdpk
  • RATCCDPK
  • wu:fj42a03
  • zgc:110275
  • calcium/calmodulin-dependent protein kinase IV-like
  • calcium/calmodulin-dependent protein kinase IV
  • CAMK4L
  • CAMK4
  • camk4
  • Camk4
Origin
Human
12
3
1
Source
Baculovirus infected Insect Cells
5
4
2
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant CAMK4.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background CAMK4 belongs to the serine/threonine protein kinase family and Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution. Sikela et al. identified that brain CaM kinase IV (CAMK4) appeared to be restricted to brain in the rat, interestingly, it was not detected in the brain of the newborn, but became detectable within a few days after birth. They also raised the possibility that a defect in Camk4 may be responsible for 1 of the mutant phenotypes in the centromeric region near 2 mutations, which known to affect neurologic function and fertility (1). Wei et al. found Camk4-labeled neurons in the hippocampus, amygdala, anterior cingulate cortex, somatosensory cortex, and insular cortex (2). Kang et al. generated transgenic mice in which the expression of a dominant-negative form of Camk4 was restricted to the postnatal forebrain. In these transgenic mice, activity-induced Creb phosphorylation and Fos expression were significantly attenuated. The results indicated that neural activity-dependent CAMK4 signaling in the neuronal nucleus plays an important role in the consolidation/retention of hippocampus-dependent long-term memory (3). Wu et al. generated transgenic mice that selectively express in skeletal muscle a constitutively active form of CAMK4 and concluded that a calcium-regulated signaling pathway controls mitochondrial biogenesis in mammalian cells (4). Wei et al. studied pain and fear memory in Camk4-null mice and concluded that Camk4 is crucial in the trapping of Ca2(+)/calmodulin complexes in neuronal nuclei and in Creb phosphorylation and activation (5).
Alternate Names: CAMK, Calcium/calmodulin-dependent protein kinase, CaM kinase-GR
Molecular Weight 79.0 kDa
Gene ID 814
UniProt Q16566
Research Area Kinases/Phosphatases
Pathways EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Production of Molecular Mediator of Immune Response, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 83 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (Active) protein (ABIN411947) Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) (Active) protein
Background publications Wei, Qiu, Liauw, Robinson, Ho, Chatila, Zhuo: "Calcium calmodulin-dependent protein kinase IV is required for fear memory." in: Nature neuroscience, Vol. 5, Issue 6, pp. 573-9, 2002 (PubMed).

Wu, Kanatous, Thurmond, Gallardo, Isotani, Bassel-Duby, Williams: "Regulation of mitochondrial biogenesis in skeletal muscle by CaMK." in: Science (New York, N.Y.), Vol. 296, Issue 5566, pp. 349-52, 2002 (PubMed).

Kang, Sun, Atkins, Soderling, Wilson, Tonegawa: "An important role of neural activity-dependent CaMKIV signaling in the consolidation of long-term memory." in: Cell, Vol. 106, Issue 6, pp. 771-83, 2001 (PubMed).

Sikela, Law, Kao, Hartz, Wei, Hahn: "Chromosomal localization of the human gene for brain Ca2+/calmodulin-dependent protein kinase type IV." in: Genomics, Vol. 4, Issue 1, pp. 21-7, 1989 (PubMed).

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