Protein Kinase A, alpha (PRKACA) (Active) Protein

Details for Product No. ABIN411950, Supplier: Log in to see
Protein Name
  • PKCD
  • Pkaca
  • PKACA
  • PKA
  • Calpha
  • Cs-PKA
  • PKCA1
  • PRKAR1
  • protein kinase, cAMP dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, catalytic, alpha
  • protein kinase, cAMP-dependent, regulatory, type I, beta
  • Prkaca
  • PRKACA
  • PRKAR1B
Origin
Human
10
1
1
1
1
1
1
1
Source
Baculovirus infected Insect Cells
6
3
2
2
2
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant PKAca.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (AMPK). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-α, C-β, and C-γ. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-α, RI-β, RII-α, and RII-β. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C-α of PKA (PKAca) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C-β of AMPK. Tasken et al. assigned the PKAca gene to 19p13.1 (1). Yasuda et al found that protein kinase A is required for long-term potentiation in neonatal tissue and suggested that developmental changes in synapse morphology may underlie the changes in the kinase activity (2). Skalhegg et al generated a null mutation in the major catalytic subunit of PKAca, and observed early postnatal lethality in the majority of C-α knockout mice. Surprisingly, a small percentage of C-α knockout mice, although runted, survived to adulthood. In these animals, compensatory increases in C-β levels occurred in brain whereas many tissues, including skeletal muscle, heart, and sperm, contained less than 10 % of the normal PKA activity (3).
Alternate Names: PKA, cAMP-dependent protein kinase catalytic subunit alpha
Molecular Weight 69.0 kDa
Gene ID 5566
NCBI Accession NM_002730
Pathways NF-kappaB Signaling, Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 137 nmol phosphate incorporated into Histone H1 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Protein Kinase A, alpha (PRKACA) (Active) Protein (ABIN411950) Protein Kinase A, alpha (PRKACA) (Active) protein
Background publications Skålhegg, Huang, Su, Idzerda, McKnight, Burton: "Mutation of the Calpha subunit of PKA leads to growth retardation and sperm dysfunction." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 3, pp. 630-9, 2002 (PubMed).

Yasuda, Barth, Stellwagen, Malenka: "A developmental switch in the signaling cascades for LTP induction." in: Nature neuroscience, Vol. 6, Issue 1, pp. 15-6, 2002 (PubMed).

Taskén, Solberg, Zhao, Hansson, Jahnsen, Siciliano: "The gene encoding the catalytic subunit C alpha of cAMP-dependent protein kinase (locus PRKACA) localizes to human chromosome region 19p13.1." in: Genomics, Vol. 36, Issue 3, pp. 535-8, 1997 (PubMed).