PKAcb (Active) Protein

Details for Product No. ABIN411951, Supplier: Log in to see
Protein Name
Origin
Human
1
Source
Baculovirus infected Insect Cells
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant PKAcb.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background Most of the effects of cAMP are mediated through the phosphorylation of target proteins on serine or threonine residues by the cAMP-dependent protein kinase (PKA). The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. The mammalian catalytic subunit has been shown to consist of three PKA gene products: C-α, C-β, and C-γ. Two PKA isoforms exist, designated types I and II, which differ in their dimeric regulatory subunits, designated RI and RII, respectively. Furthermore, there are at least four different regulatory subunits: RI-α, RI-β, RII-α, and RII-β. The cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The catalytic subunit C-β of PKA (PKAcb) is a member of the Ser/Thr protein kinase family and is a catalytic subunit C-β of AMPK. Berube et al. assigned the PKAcb to human chromosome 1 by Southern blot analysis of somatic cell hybrids (1) and Simard et al located it to 1p36.1 by in situ hybridization (2).
Alternate Names: PKA, cAMP-dependent protein kinase catalytic subunit alpha
Molecular Weight 65.0 kDa
Gene ID 5567
NCBI Accession NM_002731
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 342 μmol phosphate incorporated into CREBtide substrate per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for PKAcb (Active) Protein (ABIN411951) PKAcb (Active) protein
Activity Assay (AcA) image for PKAcb (Active) Protein (ABIN411951) PKAcb (Active) protein (Image 2)
Background publications Skålhegg, Huang, Su, Idzerda, McKnight, Burton: "Mutation of the Calpha subunit of PKA leads to growth retardation and sperm dysfunction." in: Molecular endocrinology (Baltimore, Md.), Vol. 16, Issue 3, pp. 630-9, 2002 (PubMed).

Yasuda, Barth, Stellwagen, Malenka: "A developmental switch in the signaling cascades for LTP induction." in: Nature neuroscience, Vol. 6, Issue 1, pp. 15-6, 2002 (PubMed).

Taskén, Solberg, Zhao, Hansson, Jahnsen, Siciliano: "The gene encoding the catalytic subunit C alpha of cAMP-dependent protein kinase (locus PRKACA) localizes to human chromosome region 19p13.1." in: Genomics, Vol. 36, Issue 3, pp. 535-8, 1997 (PubMed).