Protein Kinase D1 (PRKD1) (Active) Protein

Details for Product No. ABIN411952, Supplier: Log in to see
Protein Name
  • MGC83727
  • PRKD1
  • pkd
  • pkcm
  • prkcm
  • pkc-mu
  • PKC-MU
  • PKCM
  • PKD
  • PRKCM
  • PKD1
  • Pkcm
  • Prkcm
  • nPKC-D1
  • nPKC-mu
  • PKC
  • protein kinase D1
  • protein kinase D1 L homeolog
  • PRKD1
  • prkd1.L
  • prkd1
  • Prkd1
Origin
Human
9
2
Source
Baculovirus infected Insect Cells
5
2
1
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
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Purpose >90% Pure active Recombinant PKAmu.
Characteristics Protein Source: Baculovirus (Sf9 insect cells)
Purity SDS-PAGE: ≥90 %
Background Protein kinase Cmu (PKCmu) is a novel member of the protein kinase C (PKC) family that differs from the other isoenzymes in structural and enzymatic properties. It is characterized by the presence of a pleckstrin homology (PH) domain and an amino-terminal hydrophobic region and has substrate specificity distinct from other PKC isoforms. PKCmu is a ubiquitous PKC isotype with the highest expression in the thymus, lung and peripheral blood mononuclear cells (1). PKCmu forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKCmu between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2). PKCmu was also shown to associate with the B cell receptor (BCR) complex and its activity is up-regulated after cross-linking the BCR and CD19 on B cells (3). PKC mu co-precipitates with Syk and phospholipase C-γ 1/2 (PLC γ 1/2) and in vitro phosphorylation of fusion proteins showed that both Syk and PLC γ 1 are potential substrates of PKC mu in vivo. In addition, specific interaction of PKCmu and 14-3-3tau can be shown in the T cell line Jurkat by immunocoprecipitiation and by pulldown assays (4). 14-3-3tau is not a substrate of PKCmu and strongly down-regulates PKCmu kinase activity in vitro. In response to various stimuli, PKC mu activates the mitogen-activated protein kinase (p42/ERK1 MAPK cascade) but does not affect the related c-jun N-terminal kinase or p38 MAPK (5).
Alternate Names: PKC, Protein kinase C mu
Molecular Weight 131.0 kDa
Gene ID 5587
UniProt Q15139
Pathways Myometrial Relaxation and Contraction
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Activity Specification: 680 nmol phosphate incorporated into CREBTIDE substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 μM ATP (0.83 μCi/assay).

Restrictions For Research Use only
Format Liquid
Concentration 0.1 μg/μL
Buffer Recombinant proteins in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol).
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months
Supplier Images
SDS-PAGE (SDS) image for Protein Kinase D1 (PRKD1) (Active) protein (ABIN411952) Protein Kinase D1 (PRKD1) (Active) protein
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