Annexin A5 (ANXA5) Protein

Details for Product No. ABIN412007
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Protein Name
Synonyms anx, anx5, ANX V, anxa5, cb989, wu:fa98f06, wu:fj10f10, MGC89158, ANX5, ENX2, PP4, RPRGL3, Anx5, R74653, LC5, enx2
Reactivity
(35), (9), (4), (4), (4), (4), (2)
Host
(38), (9), (7), (2), (2)
Application
Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Pubmed 21 references available
Quantity 1000 tests
Options
Shipping to United States ( )
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Purpose Reagent for detecting early stages of apoptosis.
Sample Type Cell Culture
Characteristics The Annexin V-FITC conjugate can be used for detection of apoptosis by fluorescence microscopy or by flow cytometry.
Alternative Name Annexin V
Background During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis.
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
Application Notes Detection method: Flow cytometry (Ex/Em = 488/530 nm) using FL1 channel and fluorescence microscopy
Protocol A. Incubation of cells with Annexin V-FITC:
1. Induce apoptosis by desired methods.
2. Collect 1 x 10^5 cells by centrifugation.
3. Resuspend cells in 500 µL of 1X Annexin V Binding Buffer (ABIN412353).
4. Add 1 µL of Annexin V-FITC and 1 µL Propidium Iodide (ABIN412371).
5. Incubate at room temperature for 5 min in the dark.
Proceed to B or C below depending on method of analysis.

B. Quantification by Flow Cytometry:
Analyze cells by flow cytometry (Ex = 488 nm, Em = 530 nm) using FL1 channel for detecting Annexin v-FITC staining and FL2 channel for detetcing PI staining. For adherent cells, trypsinize and gently wash cells with serum-containing medium before incubation with Annexin V-FITC (A.3-5).

C. Detection by Fluorescence Microscopy:
1. Place the cell suspension from Step A.5 on a glass slide, and cover with a glass coverslip. For analyzing adherent cells, grow cells directly on a coverslip. Following incubation (A.5), invert coverslip on a glass slide and visualize cells. The cells can also be washed with 1X Annexin V Binding Buffer and fixed in 2 % formaldehyebefore visualization. (Cells must be incubated with Annexin V-FITC before fixation because any cell membrane disruption can cause nonspecific binding of annexin V to PS on the inner surface of the cell membrane.)
2. Observe the cells under a fluorescence microscope using a dual filter set for FITC and rhodamine or separate filters. Cells that have bound Annexin V-FITC will show bright green staining on the plasma membrane. Cells which have lost membrane integrity will show red staining (PI) throughout the nucleus and a halo of green staining (FITC) on the cell surface (plasma membrane).


TESTING RESULTS: Jurkat cells (treated with 2 µM camptothecin for 6 hours) were collected for annexin V assay according to the kit instructions. Results show 40-60 % apoptotic cells as analyzed by flow cytometry.
Restrictions For Research Use only
Format Liquid
Concentration 0.15 mg/mL
Buffer PBS containing 1 % BSA, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze!
Storage 4 °C
Expiry Date 12 months
Product cited in: Dondeti, Wubbenhorst, Lal et al.: "Integrative genomic analyses of sporadic clear cell renal cell carcinoma define disease subtypes and potential new therapeutic targets." in: Cancer research, Vol. 72, Issue 1, pp. 112-21, 2012 (PubMed).

Neri, Ren, Gratton et al.: "Bortezomib-induced "BRCAness" sensitizes multiple myeloma cells to PARP inhibitors." in: Blood, Vol. 118, Issue 24, pp. 6368-79, 2011 (PubMed).

Peters, Raghavan: "Endoplasmic reticulum calcium depletion impacts chaperone secretion, innate immunity, and phagocytic uptake of cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 2, pp. 919-31, 2011 (PubMed).

Sumitomo, Nakata, Higashino et al.: "Streptolysin S contributes to group A streptococcal translocation across an epithelial barrier." in: The Journal of biological chemistry, Vol. 286, Issue 4, pp. 2750-61, 2011 (PubMed).

Robbins, Tersey, Ogihara et al.: "Inhibition of deoxyhypusine synthase enhances islet {beta} cell function and survival in the setting of endoplasmic reticulum stress and type 2 diabetes." in: The Journal of biological chemistry, Vol. 285, Issue 51, pp. 39943-52, 2010 (PubMed).

Sharmeen, Skrtic, Sukhai et al.: "The antiparasitic agent ivermectin induces chloride-dependent membrane hyperpolarization and cell death in leukemia cells." in: Blood, Vol. 116, Issue 18, pp. 3593-603, 2010 (PubMed).

Wu, Zhou, Robertson et al.: "Identification of a bone marrow-derived CD8αα+ dendritic cell-like population in inflamed autoimmune target tissue with capability of inducing T cell apoptosis." in: Journal of leukocyte biology, Vol. 88, Issue 5, pp. 849-61, 2010 (PubMed).

Tsujii, Eguchi, Chenchik et al.: "Screening of cell death genes with a mammalian genome-wide RNAi library." in: Journal of biochemistry, Vol. 148, Issue 2, pp. 157-70, 2010 (PubMed).

Snyder, Reynolds, Hollingsworth et al.: "Clara cells attenuate the inflammatory response through regulation of macrophage behavior." in: American journal of respiratory cell and molecular biology, Vol. 42, Issue 2, pp. 161-71, 2010 (PubMed).

Radke, Reynolds, Melo et al.: "Mature human eosinophils express functional Notch ligands mediating eosinophil autocrine regulation." in: Blood, Vol. 113, Issue 13, pp. 3092-101, 2009 (PubMed).

Tu, Ren, Wang et al.: "The p53-cathepsin axis cooperates with ROS to activate programmed necrotic death upon DNA damage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 4, pp. 1093-8, 2009 (PubMed).

Sumi, Ishida, Okumura et al.: "Activation of tumour-necrosis factor-related apoptosis-inducing ligand receptor enhances the severity of murine allergic conjunctivitis." in: The British journal of ophthalmology, Vol. 93, Issue 1, pp. 110-5, 2008 (PubMed).

Zhang, Wang, Liu et al.: "Regulation of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by DJ-1 in thyroid cancer cells." in: Endocrine-related cancer, Vol. 15, Issue 2, pp. 535-44, 2008 (PubMed).

Vempati, Diaz, Barrientos et al.: "Role of cytochrome C in apoptosis: increased sensitivity to tumor necrosis factor alpha is associated with respiratory defects but not with lack of cytochrome C release." in: Molecular and cellular biology, Vol. 27, Issue 5, pp. 1771-83, 2007 (PubMed).

Terashima, Kojima, Fujimiya et al.: "The fusion of bone-marrow-derived proinsulin-expressing cells with nerve cells underlies diabetic neuropathy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 35, pp. 12525-30, 2005 (PubMed).

Obeng, Boise: "Caspase-12 and caspase-4 are not required for caspase-dependent endoplasmic reticulum stress-induced apoptosis." in: The Journal of biological chemistry, Vol. 280, Issue 33, pp. 29578-87, 2005 (PubMed).

Okamoto, Kawamura, Kanbe et al.: "Suppression of serum IgE response and systemic anaphylaxis in a food allergy model by orally administered high-dose TGF-beta." in: International immunology, Vol. 17, Issue 6, pp. 705-12, 2005 (PubMed).

Tyurina, Serinkan, Tyurin et al.: "Lipid antioxidant, etoposide, inhibits phosphatidylserine externalization and macrophage clearance of apoptotic cells by preventing phosphatidylserine oxidation." in: The Journal of biological chemistry, Vol. 279, Issue 7, pp. 6056-64, 2004 (PubMed).

Oshiro, Landowski, Catlett-Falcone et al.: "Inhibition of JAK kinase activity enhances Fas-mediated apoptosis but reduces cytotoxic activity of topoisomerase II inhibitors in U266 myeloma cells." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 12, pp. 4262-71, 2001 (PubMed).

Grad, Bahlis, Reis et al.: "Ascorbic acid enhances arsenic trioxide-induced cytotoxicity in multiple myeloma cells." in: Blood, Vol. 98, Issue 3, pp. 805-13, 2001 (PubMed).

Zelphati, Wang, Kitada et al.: "Intracellular delivery of proteins with a new lipid-mediated delivery system." in: The Journal of biological chemistry, Vol. 276, Issue 37, pp. 35103-10, 2001 (PubMed).

Validation Images
Catalog No. ABIN412007
1,122.00 $
Plus shipping costs $45.00
Quantity
Price
1000 tests
1,122.00 $

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)