Did you know that you can buy products from over 140 different suppliers from us?

Annexin A5 (ANXA5) Protein

Details for Product No. ABIN412010
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Protein Name
Synonyms ANX5, ENX2, PP4, RPRGL3, Anx5, R74653, LC5, anx5, enx2, anx, ANX V, anxa5, cb989, wu:fa98f06, wu:fj10f10, ANXA5, MGC89158
Reactivity
Please enquire
(35), (4), (4), (4), (4)
Host
Please enquire
(31), (5), (3), (2), (2)
Application
Flow Cytometry (FACS), Fluorescence Microscopy (FM)
Pubmed 9 references available
Quantity 200 tests
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN412010
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Purpose A bright red color fluorescent reagent for detecting of the early stages of apoptosis.
Sample Type Cell Culture
Characteristics The Annexin V-Cy3 conjugate contains the bright red fluorescent probe that can be used for detection of apoptosis by fluorescence microscopy with a rhodamine filter or by flow cytometry. Cy3 yields red fluorescence with a lambdamax emission of 570 nm.
Alternative Name Annexin V
Background During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore serves as a probe for detecting apoptosis.
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
Application Notes Detection method: Flow cytometry (Ex/EM = 543/570 nm) using FL2 channel and fluorescence microscopy
Protocol A. Incubation of cells with Annexin V-Cy3:
1. Induce apoptosis by desired methods.
2. Collect 1 x 10^5 cells by centrifugation.
3. Resuspend cells in 500 µL of 1X Annexin V Binding Buffer.
4. Add 1 µL of Annexin V-Cy3.
5. Incubate at room temperature for 5 min in the dark.
Proceed to B or C below depending on method of analysis.

B. Quantification by Flow Cytometry:
Analyze cells by flow cytometry (Ex = 543 nm, Em = 570 nm) using FL2 channel. For adherent cells, trypsinize and gently wash cells with serum-containing medium before incubation with Annexin V-Cy3 (A.3-5).

C. Detection by Fluorescence Microscopy:
1. Place the cell suspension from Step A.5 on a glass slide, and cover with a glass coverslip. For analyzing adherent cells, grow cells directly on a coverslip. Following incubation (A.5), invert coverslip on a glass slide and visualize cells. The cells can also be washed with 1X Annexin V Binding Buffer and fixed in 2 % formaldehyde before visualization. (Cells must be incubated with Annexin V-Cy3 before fixation because any cell membrane disruption can cause nonspecific binding of annexin V to PS on the inner surface of the cell membrane.)
2. Observe the cells under a fluorescence microscope using a rhodamine filter. Cells that have bound Annexin V-Cy3 will show bright red staining on the plasma membrane.
Restrictions For Research Use only
Handling Advice Do not freeze!
Storage 4 °C
Expiry Date 12 months
Product cited in: Breckenridge, Nguyen, Kuppig et al.: "The procaspase-8 isoform, procaspase-8L, recruited to the BAP31 complex at the endoplasmic reticulum." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 7, pp. 4331-6, 2002 (PubMed).

Ruiz-Vela, Opferman, Cheng et al.: "Proapoptotic BAX and BAK control multiple initiator caspases." in: EMBO reports, Vol. 6, Issue 4, pp. 379-85, 2005 (PubMed).

Zencak, Lingbeek, Kostic et al.: "Bmi1 loss produces an increase in astroglial cells and a decrease in neural stem cell population and proliferation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 24, pp. 5774-83, 2005 (PubMed).

Sato, Sanjo, Tsujimura et al.: "TAK1 is indispensable for development of T cells and prevention of colitis by the generation of regulatory T cells." in: International immunology, Vol. 18, Issue 10, pp. 1405-11, 2006 (PubMed).

Chen, Sandford, Nicholas: "Intracellular signaling mechanisms and activities of human herpesvirus 8 interleukin-6." in: Journal of virology, Vol. 83, Issue 2, pp. 722-33, 2008 (PubMed).

Kuhn, Hunsucker, Chen et al.: "Targeted inhibition of the immunoproteasome is a potent strategy against models of multiple myeloma that overcomes resistance to conventional drugs and nonspecific proteasome inhibitors." in: Blood, Vol. 113, Issue 19, pp. 4667-76, 2009 (PubMed).

Yu, Chiang, Chang et al.: "The interferon stimulator mitochondrial antiviral signaling protein facilitates cell death by disrupting the mitochondrial membrane potential and by activating caspases." in: Journal of virology, Vol. 84, Issue 5, pp. 2421-31, 2010 (PubMed).

Guan, Xie, Leithäuser et al.: "KLF4 is a tumor suppressor in B-cell non-Hodgkin lymphoma and in classic Hodgkin lymphoma." in: Blood, Vol. 116, Issue 9, pp. 1469-78, 2010 (PubMed).

Chen, Cousins, Sandford et al.: "Human herpesvirus 8 viral interleukin-6 interacts with splice variant 2 of vitamin K epoxide reductase complex subunit 1." in: Journal of virology, Vol. 86, Issue 3, pp. 1577-88, 2012 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN412010
357.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top