Enhanced Green Fluorescent Protein (eGFP) Protein

Name: Enhanced Green Fluorescent Protein (eGFP)

Source: Escherichia Coli (E. coli)

Application: Western Blotting (WB)

Catalog no.: ABIN412985

Additional Information

Description: The recombinant EGFP is expressed and purified from transformed E. coli using a method that ensures high purity and maximal GFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The recombinant EGFP is a 29 kDa monomer with 265 amino acids (Gene Bank Accession no. U57607) tagged with 6XHis on both N- and C-terminus. Therefore, the EGFP can be detected by both His-tag antibody (Cat.# 3998-100) and GFP antibody (Cat.# 3999-100) on Western Blot analysis. The excitation and emmission spectra for the recombinant EGFP is identical to GFP purified from Aequorea Victoria (Chalfie et al, 1994), with Ex/Em = 488/507 nm and ε = 55,000 M ‐1  Cm ‐1 .      .

Application Details

Application Notes: The protein is suitable as control reagent for GFP expression studies. Applications include: Use as standard for SDS-PAGE, Western blot analysis, calibration of fluorometers, flow cytometers, and fluorescence microscope, as well as microinjection of EGFP into cells and tissues, etc. The recombinant EGFP can also be conjugated to other proteins. GFP antibody is also available which recognizes the recombinant EGFP protein (Cat.# 3999-100).

Purity: >97% by SDS-PAGE and HPLC ENDOTOXIN: <0.1 ng/ μ g

Buffer: Ready to use liquid in PBS with no additives STORAGE: -80 °C for long-term storage

Restrictions: For Research Use only