4-1BBL Protein

Name: 4-1BBL

Protein Type: Recombinant

Source: Escherichia Coli (E. coli)

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN412990

Additional Information

Characteristics: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED 50 for the effect is less than 10 ng/ml. Please note: Results may vary with PBMC donors.

Description: Human 4-1BB Ligand (4-1BBL) is a member of the emerging family of Ligands with structural homology to tumor necrosis factor. Recombinant human 4-1BBL is a 19.5 kDa protein containing 185 amino acid residues, comprising the TNF-like extra-cellular domain of 4-1BBL.

Molecular Weight: 19.5 kDa

Application Details

Purity: >98% by SDS-PAGE analyses Endotoxin level is <0.1 ng per μ g.

Buffer: Lyophilized from 5 mM Tris, pH 8.0 + 75 mM NaCl.

Storage: The lyophilized protein is best-stored desiccated below 0 °C. Reconstituted human 4-1BBL should be stored in working aliquots at -20 °C. Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or –20 °C for future use.

Restrictions: For Research Use only