Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ) Protein

Name: Adiponectin, C1Q and Collagen Domain Containing (ADIPOQ)

Synonyms: ACDC, ADPN, APM1, APM-1, GBP28, ACRP30, ADIPQTL1, adiponectin, APN, Acdc, apM1, 30kDa, adipo, Acrp30, ADIPOQ, MGC138982, ADN, MGC84292

Protein Type: Recombinant

Source: Escherichia Coli (E. coli)

Origin (Gene): Rat (Rattus)

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413002

Additional Information

Characteristics: Measured by its ability to inhibit proliferation of mouse myeloid cell line M1 (Yokota, T. et al., Blood 96:1723, 2000). The ED 50 for the effect is typically 1-7 μ g/ml.

Description: Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and it secretion is stimulated by insulin. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.

Application Details

Purity: >90% by SDS-PAGE Endotoxin level is <0.1 ng per μ g.

Buffer: Lyophilized from a 0.2 μ m filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 μ g of bovine serum albumin (BSA) per 1 μ g of adiponectin.

Storage: The lyophilized protein is best-stored desiccated below 0 °C. Reconstituted Adiponectin should be stored in working aliquots at -20 °C or –70 °C. Reconstitution: We recommend a quick spin followed by reconstitution in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This stock solution can then be diluted into other aqueous buffers and stored at 4 °C for 1 week or –20 °C for future use.

Restrictions: For Research Use only