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Annexin A5 (ANXA5) Protein

Details for Product No. ABIN413009, Supplier: Log in to see
Protein Name
  • anx
  • anx5
  • ANX V
  • anxa5
  • cb989
  • wu:fa98f06
  • wu:fj10f10
  • MGC89158
  • ANX5
  • ENX2
  • PP4
  • RPRGL3
  • Anx5
  • R74653
  • LC5
  • enx2
Origin
Human
38
15
10
7
4
3
3
3
1
1
Source
Escherichia coli (E. Coli)
53
9
9
6
2
1
1
Protein Type
Recombinant
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Supplier
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Purpose >95% pure Annexin V, human recombinant, for detecting early stages of apoptosis and in competition binding experiments to compete with tagged annexin V conjugates by FACS/Western blotting.
Sample Type Cell Culture
Purification Purified
Purity SDS-PAGE: ≥98 %
Background Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The unlabeled Annexin V reagent can be conjugated to any tag of choice for detection of apoptosis. The reagent can also be used in competition binding experiments to compete with tagged annexin V conjugates. Annexin V-FITC, -Cy3, -Cy5, -EGFP, -PE, PE/CY5, and -Biotin Labels are also available separately.
Alternate Names: Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, lacental anticoagulant protein I, Thromboplastin inhibitor, Vascular anticoagulant-alpha, PP4, ANX5, ENX2, ANXA5, Annexin A5, Annexin-5, Annexin V, Lipocortin V, Endonexin II, Calphobindin I, CBP-I, Placental anticoagulant protein I, PAP-I, Placental anticoagulant protein 4, VAC-alpha, Anchorin CII.
Research Area Cardiovascular, Atherosclerosis, Apoptosis/Necrosis, Coagulation
Pathways Apoptosis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute the lyophilized forms with H₂O or PBS to a final concentration of 1-5 mg/mL.
Concentration 1 mg/mL
Buffer Liquid form (1 mg/mL in PBS, pH 7.4)
Handling Advice Centrifuge the vial prior to opening.
Storage 4 °C
Storage Comment +4°C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Annexin A5 (ANXA5) Protein (ABIN413009) Annexin A5 (ANXA5) protein
Product cited in: Bean, Ganesan, Dong et al.: "PUMA and BIM are required for oncogene inactivation-induced apoptosis." in: Science signaling, Vol. 6, Issue 268, pp. ra20, 2013 (PubMed).

Kar, Quesada: "A numerical simulation approach to studying anterior cruciate ligament strains and internal forces among young recreational women performing valgus inducing stop-jump activities." in: Annals of biomedical engineering, Vol. 40, Issue 8, pp. 1679-91, 2012 (PubMed).

Galvan, Foreman, Zeng et al.: "Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 8, pp. 3716-23, 2012 (PubMed).

Fens, van Wijk, Andringa et al.: "A role for activated endothelial cells in red blood cell clearance: implications for vasopathology." in: Haematologica, Vol. 97, Issue 4, pp. 500-8, 2012 (PubMed).

Tanaka, Jin, Lee et al.: "Hyperoxia-induced LC3B interacts with the Fas apoptotic pathway in epithelial cell death." in: American journal of respiratory cell and molecular biology, Vol. 46, Issue 4, pp. 507-14, 2012 (PubMed).

Xin, Herrmann, Reckamp et al.: "Antiangiogenic and antimetastatic activity of JAK inhibitor AZD1480." in: Cancer research, Vol. 71, Issue 21, pp. 6601-10, 2011 (PubMed).

Neniskyte, Neher, Brown: "Neuronal death induced by nanomolar amyloid β is mediated by primary phagocytosis of neurons by microglia." in: The Journal of biological chemistry, Vol. 286, Issue 46, pp. 39904-13, 2011 (PubMed).

Mao, Cao, Wood et al.: "A small-molecule inhibitor of D-cyclin transactivation displays preclinical efficacy in myeloma and leukemia via phosphoinositide 3-kinase pathway." in: Blood, Vol. 117, Issue 6, pp. 1986-97, 2011 (PubMed).

Mirza, Teo, Upcroft et al.: "A rapid, high-throughput viability assay for Blastocystis spp. reveals metronidazole resistance and extensive subtype-dependent variations in drug susceptibilities." in: Antimicrobial agents and chemotherapy, Vol. 55, Issue 2, pp. 637-48, 2011 (PubMed).

Wang, Yang, Su et al.: "Enhanced antitumor effect of combined triptolide and ionizing radiation." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 16, pp. 4891-9, 2007 (PubMed).

Medarova, Bonner-Weir, Lipes et al.: "Imaging beta-cell death with a near-infrared probe." in: Diabetes, Vol. 54, Issue 6, pp. 1780-8, 2005 (PubMed).