Annexin A5 (ANXA5) Protein

Name: Annexin A5 (ANXA5)

Synonyms: PP4, ANX5, ENX2, Anx5, R74653, LC5, MGC93655, ANXA5, MGC126971, anx, anx5, ANX V, anxa5, cb989, wu:fa98f06, wu:fj10f10, MGC89158

Protein Type: Recombinant

Host: Please enquire

Origin (Gene): Human

Application: Western Blotting (WB), ELISA, Functional Studies (Func)

Catalog no.: ABIN413011

Additional Information

Description: Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The unlabeled Annexin V reagent can be conjugated to any tag of choice for detection of apoptosis. The reagent can also be used in competition binding experiments to compete with tagged annexin V conjugates. Annexin V-FITC, -Cy3, -Cy5, -EGFP, -PE, PE/CY5, and -Biotin Labels are also available separately.
Synonyms: Annexin V, human recombinant, Anchorin CII, Annexin V, Annexin-5, Calphobindin I, Endonexin II, Lipocortin V, Placental anticoagulant protein 4, lacental anticoagulant protein I Thromboplastin inhibitor, Vascular anticoagulant-alpha

Application Details

Handling Advice: Centrifuge the vial prior to opening.

Purity: >95% by SDS-PAGE

Buffer: Liquid form (1 mg/ml in PBS, pH 7.4) 1 mg & 5 mg Lyophilized form. Reconstitute the lyophilized forms with water or PBS to a final concentration of 1-5 mg/ml.

Storage: +4°C.
Stability: 12 months

Restrictions: For Research Use only

Publications

Product: Medarova, Bonner-Weir, Lipes et al.: "Imaging beta-cell death with a near-infrared probe." in: Diabetes, Vol. 54, Issue 6, pp. 1780-8, 2005 (PubMed).

Wang, Yang, Su et al.: "Enhanced antitumor effect of combined triptolide and ionizing radiation." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 16, pp. 4891-9, 2007 (PubMed).

Mirza, Teo, Upcroft et al.: "A rapid, high-throughput viability assay for Blastocystis spp. reveals metronidazole resistance and extensive subtype-dependent variations in drug susceptibilities." in: Antimicrobial agents and chemotherapy, Vol. 55, Issue 2, pp. 637-48, 2011 (PubMed).

Mao, Cao, Wood et al.: "A small-molecule inhibitor of D-cyclin transactivation displays preclinical efficacy in myeloma and leukemia via phosphoinositide 3-kinase pathway." in: Blood, Vol. 117, Issue 6, pp. 1986-97, 2011 (PubMed).

Neniskyte, Neher, Brown: "Neuronal death induced by nanomolar amyloid β is mediated by primary phagocytosis of neurons by microglia." in: The Journal of biological chemistry, Vol. 286, Issue 46, pp. 39904-13, 2011 (PubMed).

Xin, Herrmann, Reckamp et al.: "Antiangiogenic and antimetastatic activity of JAK inhibitor AZD1480." in: Cancer research, Vol. 71, Issue 21, pp. 6601-10, 2011 (PubMed).

Tanaka, Jin, Lee et al.: "Hyperoxia-induced LC3B interacts with the Fas apoptotic pathway in epithelial cell death." in: American journal of respiratory cell and molecular biology, Vol. 46, Issue 4, pp. 507-14, 2012 (PubMed).

Fens, van Wijk, Andringa et al.: "A role for activated endothelial cells in red blood cell clearance: implications for vasopathology." in: Haematologica, Vol. 97, Issue 4, pp. 500-8, 2012 (PubMed).

Galvan, Foreman, Zeng et al.: "Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 8, pp. 3716-23, 2012 (PubMed).

Kar, Quesada: "A numerical simulation approach to studying anterior cruciate ligament strains and internal forces among young recreational women performing valgus inducing stop-jump activities." in: Annals of biomedical engineering, Vol. 40, Issue 8, pp. 1679-91, 2012 (PubMed).